离体主动脉对胰岛素介导的血管反应性作用及其机制  

Vasodilatory effect and mechanism of insulin on isolated aorta in SD rats.

作　　者：金芃芃[1] 余立群[1] 郭益民[1] 张玉青[1] 于建兴[1] 汪洋[1] 王颖[1] 

机构地区：[1]温州医学院生理学教研室,浙江温州325035

出　　处：《温州医学院学报》2005年第4期268-270,共3页Journal of Wenzhou Medical College

摘　　要：目的:探讨离体SD大鼠主动脉对一定浓度胰岛素的反应性改变及其主要机制。方法:采用血管环灌流装置,对比观察离体SD大鼠胸主动脉环在内皮存在条件下,由去甲肾上腺素(NE)引发收缩后, 未加干预或施加干预(L-NAME或5-HD抑制剂处理)对Ins反应的变化。结果:Ins(120 U/L)对NE引发收缩后的离体SD大鼠胸主动脉环具有舒张作用,血管舒张率为:(24±8)%,P<0.01,用L-NAME或5-HD抑制剂处理后,Ins对离体SD大鼠胸主动脉环的舒张作用明显减弱,血管舒张率分别为:(9±3)% 和(14±5)%,P<0.01。结论:Ins对离体SD大鼠胸主动脉环具有舒张作用,L-NAME或5-HD可阻断该舒张反应,故考虑Ins介导的血管舒张反应主要是内皮或NO依赖的舒张反应,少部分舒张反应是通过诱导mitoKATP通道开放来实现的。Objective： To investigate the vascular reactivity to the insulin on isolated aorta. Methods： The thoracic aortic rings with intact endothelium of male Sprague-Dawley rat were mounted on a bath system. Aortic rings were subjected to contractile concentration responses to NE followed by rclaxation to insulin（120 U/L）,with and without preincubation for 15 minute with L-NAME or 5-Hydroxydecanoate（5-HD）. vascular reactivety on aortic rings was measured using an isometric force transducer. Results： Insulin induced significantly vasorelaxation （percent of relaxation （24±8）%,P〈0.01）. After preincubation with L-NAME or 5-Hydroxydecanoate, both of the relaxation responses to insulin were impaired. （L-NAME （9±3）% P〈0.01 vs 5-HD （14± 5）%, P〈 0.01） Conclusion： Thc relaxatation of insulin partly is endothelium or NO-dependent, The responses was reduccd with the inhibition of mito K （ATP） channel, suggesting that the vasorelaxant mechanisms are in part dependent to mito K （ATP） channel opening.

关 键 词：胰岛素主动脉血管内皮L-NAME5-HD血管反应性 

分 类 号：R544.14[医药卫生—心血管疾病]