血红蛋白测定方法抗干扰能力的比较  

Resistance of different methods of hemoglobin assay to common interference

作　　者：程朝英 廖蓉 夏丽[2] 李生兵[3] 郭睿[3] 赵利娜[3] 廖飞[3] 

机构地区：[1]重庆市第八人民医院内科,重庆400015 [2]重庆医科大学附属第一医院 [3]重庆医科大学生物化学教研室,重庆400016

出　　处：《重庆医科大学学报》2005年第4期591-593,共3页Journal of Chongqing Medical University

基　　金：重庆市教委高校首批优秀中青年骨干教师(廖飞)项目;重庆市科委应用基础项目(2002-7305)

摘　　要：目的:探讨抗氧化体系干预物质和维生素C(VC)对血红蛋白(Hb)测定的干扰。方法:兔红细胞溶血后为样品。比较抗氧化酶抑制剂和VC对直接测定Hb、测定碱化Hb、测定叠氮Hb等的干扰。结果:直接测定Hb吸收、测定叠氮Hb都受到VC、超氧化物歧化酶抑制剂、过氧化氢酶抑制剂等的干扰,碘乙酰胺和谷胱甘肽过氧化物酶抑制剂干扰较小。上述物质对测定碱化Hb几乎没有干扰。结论:测定碱化Hb用于评价高浓度VC的溶血作用和机制更可靠。Objective：To investigate the effects of common inhibitors of the antioxidation system and vitamin C on the measurement of hemoglobin （Hb）. Methods ： Hemolyzed rabbit red blood cell was used as the sample. The interferences of common inhibitors of theantioxidation system on assay of Hb by its absorbance at 540nm, alkalinized Hb by absorbance at 575nm, the azide- Hb complex by absorbance at 542nm were compared. Results ：Vitamin C and the inhibitors of superoxide dismutase, catalase exhibited larger interferences with direct as.say of Hb by abmrbance at 540 nm and the assay of azide - Hb, and inhibitor of GSH and GSH peroxidase showed smaller effects. The axsay of alkalinized Hb exhibited the strongest resistance to all these chemicals. Conclusion ： The assay of alkalinized Hb shows the strongest resistance to common chemicals used to investigate the mechanism of peroxidative damage and can be applicable to the study of the mechanism of vitamin C- induced hemolysis.

关 键 词：血红蛋白测定 维生素C 抗氧化体系 碱化血红蛋白 溶血 

分 类 号：Q503[生物学—生物化学]