毛细管电泳手性分离-激光诱导荧光检测普萘洛尔对映体  

Chiral Separation and Laser-Induced Fluorescence Detection of Propranolol Enantiomers by Capillary Electrophoresis

作　　者：王伟[1] 陈宇云[1] 杨维平[1] 章竹君[1] 

机构地区：[1]陕西师范大学化学与材料科学学院,西安710062

出　　处：《分析化学》2005年第8期1113-1115,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No.20175039)

摘　　要：用异硫氰酸荧光素衍生普萘洛尔,衍生产物用毛细管电泳分离,激光诱导荧光方法检测.在含有10 mmol/L β-CD,50 mmol/L 硼砂(pH=9.0)的溶液中,普萘洛尔对映体得到了基线分离.本方法线性范围宽(R型:2.0×10-3～1 mg/L), 灵敏度高(检出限均为1.24 μg/L),可用于活体中普萘洛尔对映体的药代动力学研究.Propranolol （PPL） can react with fluorescence-5-isothiocyanate （FITC） in an appropriate condition, then derivative FITC-PPL is achieved. FITC-PPL was baseline separated using 10 mmol/L β-cyclodextrin as chiral selector and 50 mmol/L borate solution as buffer. The uncoated fused-silica capillary of 75μm i. d.and 57 cm （effective length 50 cm） was used. The capillary temperature was maintained at 20℃. Samples were inejcted with pressure at 3.45 kPa for 3 s and separated at 18 kV. Both R-PPL and S-PPL had low detection limits （ 1.24μg/L）. The linear range of R-PPL was 2.0 × 10^-3 - 1.0 mg/L. The method offers a high sensitivity and broad linear range, and it can be used to do the research of the pharmacokinetics of PPL enantiomers.

关 键 词：毛细管电泳 手性分离 激光诱导荧光 普萘洛尔 对映体 Β-受体阻滞剂 药物分析 

分 类 号：TQ460.7[化学工程—制药化工]