pH荧光纳米传感器的研制及表征  

Development and Characterization of Novel Fluorescence pH Nanosensor

作　　者：梁建功[1] 宋春华[1] 何治柯[1] 

机构地区：[1]武汉大学化学与分子科学学院,武汉430072

出　　处：《分析化学》2005年第8期1119-1121,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20275028);"863"计划(No.2002AA2Z2004)资助项目

摘　　要：利用溶胶-凝胶技术制得了单分散性很好的二氧化硅纳米微球,通过共价偶联方式引入对pH变化敏感的荧光素异硫氰酸酯(FITC),这种FITC功能化二氧化硅纳米微球(FITC功能化硅微球)可作为pH荧光纳米传感器。当pH值从4.0变至7.5时,荧光强度增大约30倍。该传感器灵敏度高,响应速度快,容易制备,光稳定性好,可望用于单细胞的pH分析。Mono-dispersed silicon dioxide spherical nanoparticles have been prepared by sol-gel method and a novel fluorescence pH nanosensor was developed with surface modification of fluorescein isothiocyanate（FITC）. The nanosensor is sensitive over the pH range 4.0 - 7.5. When the pH value changes from 4.0 to 7.5, the fluorescence intensity of this nanosensor increases about 30 times. As a pH sensor, it has several unique advantages in high sensitivity, rapid response times, easy preparation and good photostability. It can also be further used as pH probe in single-cell analysis.

关 键 词：pH荧光纳米传感器 研制 表征 溶胶-凝胶法 二氧化硅纳米微球 表面修饰 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]