实时荧光定量PCR与酶联定量PCR技术在HBV-DNA载量检测中的比较研究  

Comparative study of RQ-PCR and ELQ-PCR in the detection of HBV-DNA load

作　　者：马力[1] 赵桂珍[1] 

机构地区：[1]中国医科大学附属第二医院感染病实验室,辽宁沈阳110004

出　　处：《中国医科大学学报》2005年第4期341-343,共3页Journal of China Medical University

摘　　要：目的:比较实时荧光定量PCR(RQ-PCR)与酶联定量PCR(ELQ-PCR)技术在检测HBV感染血清HBV-DNA载量的灵敏度,精确性。为乙型肝炎临床抗病毒治疗和评价药物疗效提供可靠的依据。方法:应用RQ-PCR方法检测212例(4组)HBV感染者血清,ELQ-PCR方法检测228例(4组)HBV感染者血清。结果:RQ-PCR和ELQ-PCR方法检测HBV-DNA,其阳性检出率分别为HBsAg,HBeAg,HBcAb-IgG(+)组100%和82.61%;HBsAg,HBeAb,HBcAb-IgG(+)组90.63%和73.21%;HBsAg,HBcIgG,HBcAb-IgM(+)组80.00%和57.58%;HBsAg,HB-cAb-IgG(+)组70.15%和51.43%。HBV-DNA载量的阳性检出率,经统计学处理有显著性差异(P<0.05)。结论:RQ-PCR方法检测HBV-DNA载量在实时监测,节时快速,高灵敏,高精确度等方面都更优于ELQ-PCR。Objective： To compare the sensitivity and accuracy of RQ-PCR and ELQ-PCR in detecting the HBV-DNA load and to provide a reliable basis for clinical antiviral therapy and the evaluation of the curative effect of drugs.Methods：Two-hundred and Twelve and 228 serum samples with positive HBV markers were detected by RQ-PCR and ELQPCR, respectively. Both the samples detected by RQ-PCR and ELQ-PCR were equally divided into 4 groups ： group 1 with HBsAg（ ＋ ）, HBeAg（ ＋ ）,HBcAb-IgG（ ＋ ）, group 2 with HBsAg（ ＋ ）, HBeAb（ ＋ ）, HBcAb-IgG（ ＋ ）, group 3 with HBsAg（ ＋ ）, HBcIgG（ ＋ ）, HBcAb-IgM （ ＋ ）, and group 4 with HBsAg（ ＋ ）, HBcAb-IgG（ ＋ ）. Results：The positive rate of HBV-DNA detected by RQ-PCR and ELQ-PCR in 4 groups were 100.00% and 82.61%, 90. 63% and 73.21%，80. 00% and 57.58%, and 70. 15% and 51.43% （ P 〈 0.05 ）. Conclusion：The sensitivity and accuracy of RQ-PCR are superior to those of ELQ-PCR in detecting the HBV-DNA load.

关 键 词：实时定量PCR 酶联定量PCR 乙型肝炎病毒核酸 

分 类 号：R373.2[医药卫生—病原生物学]