玉米抗矮花叶病QTL定位  

Identification of Quantitative Trait Loci Conferring Resistance to Sugarcane Mosaic Virus in Maize

作　　者：陈旭[1] 李新海[1] 郝转芳[1] 王振华[2] 田清震[1] 李明顺[1] 白丽[1] 张世煌[1] 

机构地区：[1]中国农业科学院作物科学研究所农业部作物遗传育种重点开放实验室 [2]东北农业大学,黑龙江哈尔滨150030

出　　处：《作物学报》2005年第8期983-988,共6页Acta Agronomica Sinica

基　　金：国家863计划(2003AA207070);国际科学基金(C31941);亚洲玉米生物技术协作网项目资助。

摘　　要：利用玉米自交系X178(抗)和B73(感)组配的F2群体(234个单株),构建了包含249个SSR和AFLP标记位点的遗传连锁图谱,图谱全长1659.3cM,平均图距6.58cM。采用人工接种法对216个F3家系在苗期、拔节期和抽雄期分别进行玉米矮花叶病的抗性鉴定。应用复合区间作图法分析抗病QTL与基因效应。结果表明,在3个发育阶段检测到的抗病QTL数目及作用方式略有不同。在苗期和拔节期各检测到4个抗病QTL,分别位于第3、5、6和9染色体上,可解释总表型变异的53.9%和54.8%;在抽雄期检测到5个抗病QTL,位于第2、3、5、6和9染色体上,可解释总表型变异的65.1%。在3个发育阶段第5染色体上的抗病QTL均表现为主效位点,可解释表型变异的26.1%～40.5%。Maize dwarf mosaic disease caused by sugarcane mosaic virus （SCMV） is a worldwide disease and is also one of the most important diseases in China Corn Belt. Developing disease-resistant cultivars is the most economic and efficient approach to control the disease. To explore SCMV-rcsistant genes and the linked markers in Chinese maize germplasm by molecular marker technology will offer useful information for setting strategy to facilitate disease-resistant breeding efficiently. In this study, the genetic linkage map was constructed with 249 SSR and AFLP markers based on a maize population consisting of 234 F2 individuals of the cross X178 （resistant） × B73 （susceptible）, covering 1 659.3 cM on total ten chromosomes with an average interval length of 6.58 cM. The population of 216 F3 families was evaluated for resistance to SCMV under artificial inoculation at three growth stages （ i.e. seedling, elongation and anthesis stages）. The results showed that the number of QTL detected for SCMV resistance and gene action were a little different. With the method of composite interval mapping, 4 QTL conferring resistance to SCMV were detected on chromosomes 3, 5, 6 and 9 at seedling and elongation stages, explaining 53.9% and 54.8% of total phenotypic variance; 5 QTL identified on chromosomes 2,3, 5, 6 and 9 at anthesis stage, accounting for 65. 1% of phenotypic variance, respectively. One major QTL on chromosome 5 was consistently detected at three growth stages, explaining 26.1% to 40.5 % of the phenotypic variance。

关 键 词：玉米 甘蔗花叶病毒 遗传连锁图谱 数量性状位点 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]