家蚕Bm Tpi基因Z染色体定位  

Localization of the Tpi gene on Z chromosome of the silkworm, Bombyx mori by real-time quantitative PCR

作　　者：牛宝龙[1,2] 吕顺霖[1] 翁宏飚[3] 沈卫锋[3] 孟智启[3] 

机构地区：[1]浙江大学动物科学学院 [2]浙江省农业科学院蚕桑研究所,杭州310021 [3]浙江省农业科学院蚕桑研究所

出　　处：《昆虫学报》2005年第4期622-626,共5页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30371087)

摘　　要：采用实时定量PCR技术,以家蚕Bombyxmori第11号染色体上的DH-PBAN基因为参照基因,检测家蚕不同个体间BmTpi基因与常染色体上DH-PBAN基因的拷贝数之比,雄体BmTpi∶DH-PBAN=1.0,雌体BmTpi∶DH-PBAN=0.5;并用已经定位于Z染色体上的BmKettin基因为参照,检测BmTpi基因的拷贝数与BmKettin基因的拷贝数之比,雄体BmTpi∶BmKettin=1.0,雌体BmTpi∶BmKettin=1.0,证明BmTpi基因在家蚕基因组中的拷贝数与BmKettin基因相同,说明BmTpi基因位于Z染色体上。The ratio of copy number between Bm Tpi and DH-PBAN, which is located on the llth chromosome was detected with the real-time quantitative PCR technique. It was 1.0 in males and 0.5 in females. The ratio of copy number between Bm Tpi and Bm Kettin, which is located on Z chromosome, was same in males and females. This indicated that the copy number of Bm Tpi was equal to that of Bm Kettin in the silkworm genome, i. e. one copy in females and two in males. The results suggest that Bm Tpi is located on Z chromosome in the genome of silkworm.

关 键 词：家蚕Bm Tpi基因 DH-PBAN基因 BM Kettin基因 Z染色体 实时定量PCR 

分 类 号：Q966[生物学—昆虫学]