家蚕催青后期胚胎蛋白质双向电泳图谱分析  

Analysis of protein patterns from embryo of silkworm Bombyx mori at later stages by two dimensional polyacrylamide gel electrophoresis

作　　者：钟伯雄[1] 陈金娥[1] 颜新培[1] 徐孟奎[1] 梁建设[2] 

机构地区：[1]浙江大学动物科学学院生物资源科学系,杭州310029 [2]浙江大学生物化学有限公司,杭州310029

出　　处：《昆虫学报》2005年第4期637-642,共6页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30271004);国家重点基础研究发展规划"973"项目(2005CB121003)

摘　　要：采用蛋白质双向电泳技术分析了家蚕Bombyxmori催青后期胚胎蛋白质图谱的变化。研究发现:在头胸分化期(戊3)、反转期(己1)、毛瘤发生期(己2)、点青期(己3)、转青期(己4)和孵化期(己5)胚胎蛋白质的双向电泳图谱中共检测到209个特异蛋白斑点,其中己3和己4两个胚胎出现的特异蛋白斑点数在整个催青期胚胎中为最多,分别达55和77个。与催青前期胚胎出现的特异蛋白斑点变化规律相似,这些特异蛋白斑点大多也是在随后邻近的胚胎发育中消失。推测这些特异蛋白可能与相应胚胎的形体特征发育有关。Using two dimensional polyacrylamide gel electrophoresis （2D-PAGE） and image analysis system, the changes of the proteins from later stages （after the shortening stage） of embryo of silkworm Bombyx mori were analyzed to discover the protein changing pattern during embryo development. A total of 209 specific protein spots were found in the 2D-PAGE pattern of embryos at the head thorax differentiation, reverse, tubercle appearance, head pigmentation, body pigmentation and hatch stages. Among them, the specific protein spots expressed in the head pigmentation stage and body pigmentation stage embryo contributed the largest quantity, namely 55 and 77 respectively. Similar to the changing pattern of the specific protein spots of embryo at the earlier stages, most of these specific protein spots disappeared in the embryos shortly afterwards. This suggested that those specific proteins might be related to the corresponding body characteristics in embryo development.

关 键 词：家蚕 蛋白质 双向电泳 图谱分析 胚胎发育 

分 类 号：S881.2[农业科学—特种经济动物饲养]