FK506促进乳鼠雪旺细胞增殖的实验研究  

Experimental study of proliferation of Schwann cells cultured with FK506 in vitro

作　　者：张振伟[1] 武雷[2] 杨俊[2] 陈泽华[1] 张家俊[1] 庄加川[1] 廖坚文[1] 林冷[1] 秦建强[2] 

机构地区：[1]深圳市沙井人民医院手外科,518104 [2]广州市南方医科大学临床解剖学研究所,广东省组织构建与检测重点实验室,518104

出　　处：《中华手外科杂志》2005年第4期249-251,共3页Chinese Journal of Hand Surgery

基　　金：国家自然科学基金资助项目(39970833);广东省科技攻关基金资助项目(2003C33812);广东省科技计划专项(2003A3020101);深圳市科技计划项目(200204179)

摘　　要：目的观察FK506对体外培养的乳鼠雪旺细胞增殖的影响。方法将纯化的雪旺细胞分两组,一组设为对照,另一种加含终浓度为0.5ng/mlFK506的DMEM/F12培养液培养,用MTT法检测不同时间点的OD值并绘制生长曲线,另在培养第48、72小时后用3H-胸腺嘧啶核甙测定法检测其DPM值。结果经含0.5ng/mlFK506培养液培养的雪旺细胞,其对数生长期提前出现,并且在峰值上高于对照组;接种后48h和72h,3H-胸腺嘧啶核甙检测DPM值实验组也高于对照组,差异有统计学意义(P<0.05)。结论FK506有促进雪旺细胞分裂增殖的作用,可能是其促进周围神经再生的重要机制之一。Objective To investigate the effects of FK506 on the proliferation of Schwann cells cultured in vitro. Methods Purified Schwann cells were cultured under different conditions according to group assignment. In the experiment group, Schwann cells were cultured in DMEM/F12 media supplemented with 0.5ng/nd FK 506, while in the control group the cells were cultured in the same culture media without FK 506. The OD of different intervals was determined by MTT assay to obtain the growth curves. Thymidine incorporation assay was applied after 48 hours and 72 hours of culture respectively to determine the DPM value. Results The logarithmic growth phase of Schwann cells appeared earlier in the experiment group than in the control. The peak value was also higher than that of the control group. The DPM of Schwann cells cultured with FK506 for 48 hours and 72 hours was higher than that of those cultured without FK506. All those differences were statistically significant. Conclusion FK506 can enhance the proliferation of Schwann cells cultured in vitro. This chould be one of the underlying mechanisms of its effect for promoting nerve regeneration.

关 键 词：FK506 乳鼠 雪旺细胞 细胞增殖 周围神经 

分 类 号：R651.3[医药卫生—外科学]