2-氯化-n-氨基葡萄糖乙酰基三苯基季膦盐与DNA作用的荧光光谱研究及其应用  

The Fluorescence Studies of Interaction of 2-(n-2'-Glucosyl) Actylramidyl Triphenyl Phosphonium Chloride with DNA and Its Application

作　　者：刘立明[1] 蔡朝霞[1] 宋功武[1] 娄兆文[1] 何瑜[1] 

机构地区：[1]湖北大学化学与材料科学学院,武汉430062

出　　处：《分析科学学报》2005年第4期367-370,共4页Journal of Analytical Science

基　　金：湖北省教育厅基金(No.2003A008)

摘　　要：本文合成了2-氯化-n-氨基葡萄糖乙酰基三苯基季膦盐( 2-GATPC ).利用荧光光谱法研究了2-GATPC与DNA的作用.实验结果表明,在Ph 3左右,DNA以静态猝灭的方式使2-GATPC的荧光强度显著降低. DNA的浓度在1.6×10-6～4.8×10-5 mol·L-1的范围内,对2-GATPC的荧光有良好的线性猝灭关系,据此可以测定DNA,检出限可达8.4×10-7 mol·L-1.方法简便快速,背景干扰小,对合成样品中DNA的测定,结果令人满意.A new compound 2-（n-2＇-glucosyl） actylramidyl triphenyl phosphonium chloride （2-GATPC） was synthesized, and its interaction with nucleic acid DNA was studied by fluorescence method. The results of the experiment indicated that DNA obviously decreased the fluorescence intensity of 2-GATPC by the static quenching style around pH 3. DNA could be determined by using the fluorescence quenching action of DNA on 2-GATPC. The linear range was 1.6 × 10^-6 ～ 4.8 × 10^-5 mol·L^-1 with the detection limit of 8.4 × 10^-7 mol·L^-1. The effects of interference substances and optimization of procedure were investigated. Three synthetic samples were determined satisfactorily.

关 键 词：2-氯化-n-氨基葡萄糖乙酰基三苯基季膦盐 荧光光谱 静态猝灭 DNA 

分 类 号：O657.39[理学—分析化学] Q523[理学—化学]