免疫载药毫微粒对晶状体上皮细胞的体外抑制作用  

Inhibition of LECs in vitro by drug-loaded immunonanoparticles

作　　者：刘新玲 李筱荣[1] 孙靖[1] 袁佳琴[1] 刘小燕[2] 常津[2] 

机构地区：[1]天津医科大学眼科中心,300070 [2]天津大学材料学院纳米生物研究所

出　　处：《眼科研究》2005年第4期347-349,共3页Chinese Ophthalmic Research

基　　金：国家自然科学基金(503730333);国家重大基础研究(973);预研基金(2001CCC01400);天津市自然科学基金(013616611;003702711);天津市科委重点科技攻关项目(023111711)资助

摘　　要：目的制备免疫载药毫微粒HILE6-PLA(5-Fu)-NP,体外评价其表征、免疫学特性及对晶状体上皮细胞(LECs)增殖的抑制作用。方法将抗人LECs膜单克隆抗体HILE6与5-Fu聚乳酸毫微粒采用碳二亚胺法相连接制备免疫毫微粒。扫描电镜观察微粒形态;间接免疫荧光法评价其免疫特异性;建立LECs、角膜基质细胞培养模型,体外检测不同质量浓度免疫微粒和单纯载药微粒对细胞作用2h后的杀伤作用。结果免疫毫微粒保持对LECs的免疫特异性,对LECs具有很强的杀伤作用,明显高于同质量浓度毫微粒,62·5μg/ml作用2h抑制率可达到92·3%,撤药后观察时间延长到7d时的抑制作用高于或不低于3d时的效果。使用药物质量浓度<12·5μg/ml对角膜细胞相对较安全。结论该免疫毫微粒可有选择性的,长时间杀伤LECs,预防后囊混浊。Objective To prepare an immunonanoparticles HILE6-PLA （5-Fu）-NP and evaluate the immunological characters and the toxicity to lens epithelial cells （LECs） in vitro. Methods The immunonanoparticles were made by crosslinking LEC monoclonal antibody HILE6 to PLA nanopartieles loading 5-fluorouracil PLA （5-Fu）-NP. The morphology of HILE6-PLA（5-Fu）-NP was observed by scanning electrical microscope. The immunological characters of HILE6-PLA （5-Fu）-NP was analyzed by indirect immunofluorescenee. LECs and cornea stroma cell culture system were used for the testing of the cytotoxity of HILE6-PLA（5-Fu）-NP and PLA（5-Fu）-NP with different dosage in vitro and divided into 3 groups： LECs with PLA （5-Fu）-NP（ Group A ） ;LECs with HILE6-PLA（5-Fu）-NP（ Group B ） ;cornea stroma cell with HILE6-PLA （5-Fu）-NP（ Group C ）. Results HILE6-PLA（5-Fu）-NP remained the immunological activity and had stronger potential to inhibit LECs proliferation than PLA （5-Fu）-NP at the same concentration, A 92, 3% inhibition was observed at 2 hours after exposure of LECs to 62. 5μg/ml of immunonanoparticles. The inhibitory effect at the 7th day was equal to or better than that at the 3rd day after withdrawal of the HILE6-PLA（5-Fu）-NP, 12.5μg/ml or below HILE6-PLA （5-Fu）-NP had no obvious effect on the cornea stroma cells,Conclusion The HILE6-PLA （5-Fu）-NP can prevent posterior capsular opacification through selectively and long-time killingLECs.

关 键 词：免疫纳米毫微粒 晶状体上皮细胞 单克隆抗体 靶向治疗 后囊膜混浊 

分 类 号：R96[医药卫生—药理学]