成骨细胞与血管内皮细胞联合培养对成骨细胞功能的影响  

Influence of Vascular Cell Co-cultured with Osteoblast in vitro on Ability of Osteoblast

作　　者：张健[1] 胡敏[1] 张文怡[2] 

机构地区：[1]解放军总医院口腔颌面外科,北京100853 [2]锦州医学院附属口腔医院口腔颌面外科,辽宁锦州121000

出　　处：《华西口腔医学杂志》2005年第4期325-328,共4页West China Journal of Stomatology

基　　金：全军"十五"医药卫生科研基金面上课题资助项目(01MA111)

摘　　要：目的探讨不同比例兔成骨细胞(ROB)与血管内皮细胞(RVEC)直接联合共培养后,对成骨细胞功能的影响。方法取兔成骨细胞与血管内皮细胞,将两者分为4组分别以1∶0、1∶1、2∶1、4∶1直接联合共培养于培养皿中,通过对细胞内碱性磷酸酶活性(ALP)测定和培养液中骨钙素(OC)定量测定,观察血管内皮细胞对成骨细胞功能的影响。结果成骨细胞与血管内皮细胞直接联合共培养相容性良好,2∶1的细胞比例组ALP活性及OC含量较其他各组明显增高。结论在体外直接联合共培养的体系中,血管内皮细胞有明显促进成骨细胞活性的作用。Objective The purpose of this study was to observe the influence on proliferation of rabbit osteoblast （ROB） of rabbit vascular endothelial cell（RVEC） co-cultured with rabbit osteoblast （ROB） .Methods Rabbit osteoblasts and rabbit vascular endothelial cells in ratio of 1：0,1 ： 1,2：1,4： lwere selected to co-culture.The function of osteoblasts was assessed by alkaline phosphatase（ ALP） activity and osteocalcin（ OC） assay. Results There was good compatibility when osteoblasts and vascular endothelial cells were co-cultured. The activity of ALP and OC in the group of ROB and RVEC co-cultured in ratio of 2：1 was higher than that of the other three groups. Conclusion In direct co-culture system in vitro, RVEC can improve activity of ROB.

关 键 词：成骨细胞 血管内皮细胞 碱性磷酸酶 骨钙素 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]