赤点石斑鱼精子发生和形成的超微结构研究  

Ultrastructure of Spermatogenesis and Spermiogenesis in Red - spotted Grouper (Epinephelus akaara)

作　　者：舒琥[1,2] 刘晓春[1] 张勇[1] 林浩然[1] 

机构地区：[1]中山大学水生经济动物研究所//广东省水生经济动物良种繁育重点实验室 [2]广州大学生物与化学工程学院生物系,广东广州510405

出　　处：《中山大学学报（自然科学版）》2005年第4期103-106,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家海洋863项目(2001AA621010)国家自然科学基金项目(39970586)广东省科技计划项目(A3050201;2003C20308)教育部科学技术研究重点资助项目(02150)

摘　　要：用透射电镜研究了赤点石斑鱼Epinepheluskaara精子发生和精子形成过程。结果表明,赤点石斑鱼精子发生经历了初级精原细胞、次级精原细胞、初级精母细胞、次级精母细胞、精子细胞阶段。精子细胞再经过精子形成过程成为精子。在初级精原细胞、次级精原细胞阶段,细胞核释放出拟染色体。拟染色体分布于精原细胞和前期Ⅰ的初级精母细胞的细胞质中。初级精母细胞减数分裂早期同源染色体配对明显可见,并形成联会复合体。精子细胞之间存在细胞联系(桥粒)。赤点石斑鱼成熟精子的特点是细胞核圆形,核内染色质致密,没有核泡(核空隙)。精子尾部细长,横切面为典型的“9+2”轴丝结构。The ultrastructure of spermatogenesis and spenniogenesis in red spotted grouper （Epinephelus akaara） is investigated by means of transmission electron microscope. The results show that the spermatogenic process included following stages primary spermatogonia, secondary spermatogonia , primary spermatocytes, secondary spermatocytes, spermatids and formation of sperm. At the stages of primary and secondary spermatogonia, nuclei released chromatoid bodies, which spread through the cytoplasm of spermatogonia and primary spermatocytes of prophase Ⅰ . The homologous chromosomes started pairing and forming synaptonemal complex in the primary spermatocytes . There exist cell junctions（desmosome） between the two spermatids . Matured spermatozoon is composed of head, midpeiece and tail. The head has a round-like nucleus with a nuclear. There is not any acrosome in front of nucleus . The tail is long and there is a pair of lateral fins along the two sides of flagellum . The flagellum shows typical axoneme organization of “9 ＋ 2”.

关 键 词：赤点石斑鱼 精子发生 精子形成 超微结构 

分 类 号：Q959[生物学—动物学]