反相高效液相色谱法测定珍珠梅中芦丁的含量  

Study on Determination of Rutin in Sorbaria sorbifolia by RP-HPLC Assay

作　　者：关丽萍[1] 金晴昊[1] 郑云花[1] 张学武[2] 

机构地区：[1]延边大学药学院,吉林延吉133000 [2]延边大学医学院,吉林延吉133000

出　　处：《时珍国医国药》2005年第8期717-718,共2页Lishizhen Medicine and Materia Medica Research

基　　金：吉林省延边朝鲜族自治州科技发展计划项目(No.2003YBKJ027)

摘　　要：目的建立珍珠梅中芦丁含量的测定方法。方法采用YMC-packODS-A(4.6mm×250mm,5μm)色谱柱,流动相为甲醇水溶液(35∶65);柱温25℃;流速0.4ml·min1。检测波长为256nm。结果此法线性范围1.0～6.0μg,r=0.9990。平均回收率为98.8%,RSD为1.15%(n=5)。结论测定方法简便,精密度高,准确性好,可以作为珍珠梅中黄酮的测定方法。Objective： To establish a RF-HPLC method for the determination of rutin in Sorbaria sorbifolia.Methods：YMC-pack ODS-A（4.6 mm×250mm,5μm） column was used. The mobile phase consisted of methanol-water（35 ：65） with flow rate 0.4 ml·min^-1 and column temperature at 25 ℃ ,at detection wavelength of 256 nm. Results：The method was liner within the range of 1.0～6.0μg（r=0.999 0）. The average recovery of assay was 98.8% and RSD 1.15%（n=5）. Conclusion：The method appeared to be simple and rapid, highly accurate and precise. It can used for the quantitative determintion of flavone in Sorbaria sorbifolia.

关 键 词：珍珠梅 反相高效液相色谱法 芦丁 含量测定 

分 类 号：R284.2[医药卫生—中药学]