微乳液介质-配合物光谱探针高灵敏光度法测定微量蛋白质的研究  

Studies on the Determination of Protein with Spectroscopic Probe of o-Chlorophenylfluorone-Molybdenum(Ⅵ) Complex in Microemulsion Medium

作　　者：杜斌[1] 李燕[1] 张慧[1] 吴丹[1] 魏琴[1] 李在均 

机构地区：[1]济南大学化学化工学院,山东济南250022 [2]江南大学化学与材料工程学院,江苏无锡214036

出　　处：《光谱学与光谱分析》2005年第7期1110-1113,共4页Spectroscopy and Spectral Analysis

基　　金：山东省自然科学基金(Y2000B09);山东省计划项目(03C05)资助

摘　　要：研究了在OP微乳液介质中钼(Ⅵ)-邻氯苯基荧光酮(o-CPF)-蛋白质体系的相互作用和吸收光谱情况. 在最佳条件下, 体系的摩尔吸光系数ε533 nm=6.12×106 mol-1·cm-1, 牛血清蛋白质含量在0～14 μg·mL-1范围内服从比尔定律; 同时采用摩尔比法和斜率比法测得配合物与蛋白质的结合数n=91. 初步探讨了蛋白质与o-CPF-Mo(Ⅵ)配合物相互作用机理. 将OP微乳液引入到蛋白质的测定中, 显著地提高了体系的灵敏度. 实验结果表明: 此法具有很高的灵敏度、选择性和稳定性, 可直接用于尿样的定量测定.The interaction and absorption spectral behavior of o-chlorophenylfltiorone （o-CPF）-Mo（Ⅵ） and protein are studied in OP microemulsion medium. Under the optimum condition, the molar absorptivity of o-CPF-Mo（Ⅵ）-BSA system at 532nm is 6.12×10^6L· mol^-1·cm^-1, and Beer＇ s law is obeyed in the range of 0-14 μg· mL^-1 of BSA. Moreover, the binding number between the complex and BSA determined by molar ratio and slope ratio methods is 91. The mechanism of interaction of BSA and o-CPF-Mo（Ⅵ） is also discussed. OP microemulsion media introduced into the protein determination increased markedly the sensitivity of the system. The result of experiment indicates that the method possesses a high sensitivity as well as a high selectivity. It can be used to determine protein in human urine with satisfactory results.

关 键 词：蛋白质 微乳液 钼(Ⅵ)-o-CPF配合物 光谱探针 

分 类 号：O657.3[理学—分析化学]