新型快速显微多道分光光度技术对血红蛋白的光谱分析  

Spectrum Analysis of Erythrocyte Intracellular Hemoglobin with a Novel Fast Multi-Channel Micro-Spectrophotometry

作　　者：阮萍[1] 黄耀熊[2] 李丹[3] 

机构地区：[1]桂林医学院物理教研室,广西桂林541004 [2]暨南大学生物医学工程研究所,广东广州510632 [3]广西师范大学物理与信息工程学院,广西桂林541004

出　　处：《光谱学与光谱分析》2005年第7期1121-1124,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(30227001)资助项目

摘　　要：用新型快速显微多道分光光度(MMSP)技术,在生理环境(pH、温度、氧分压和渗透压)改变下,对单个活态人血红细胞内血红蛋白分子的吸收光谱进行监测。发现生理环境的改变,引起血红蛋白吸收光谱曲线540,575nm特征吸收峰的峰高、峰位和峰形发生了不同程度的变化。MMSP技术提示血红蛋白分子的结构、浓度和功能与上述生理环境密切相关。With the application of a novel fast multi-channel micro-spectrophotometry（MMSP）, the absorption spectrum of intracellular hemoglobin molecule in a human single living red blood cell was monitored under the altered physiological environments （pH, temperature, partial pressure of oxygen, and osmotic pressure）. It was found that on the absorption spectrum curve of hemoglobin the spike, the height and the position of the characteristic absorption peaks at 540 and 575nm were altered obviously with the changes in physiological conditions, indicating that the concentration, structure and function of the hemoglobin in the red blood cell may be closely associated with the physiological environments.

关 键 词：快速显微多道分光光度技术 血红蛋白 吸收光谱 氧分压 渗透压 

分 类 号：Q27[生物学—细胞生物学] Q657.3