HPLC测定回生口服液中大黄素和大黄酚的总含量  

Content determination of emodin and chrysophanol in Huisheng oral solution by HPLC

作　　者：邓晓鸿[1] 董林[1] 尹靖先[1] 车晓彦[1] 

机构地区：[1]四川省药品检验所,四川成都610036

出　　处：《华西药学杂志》2005年第4期349-351,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立回生口服液中大黄素和大黄酚的含量测定方法.方法采用HPLC法.用Akzonobel Kromasil C18色谱柱,以甲醇-0.07%磷酸溶液(70∶30)为流动相,检测波长254 nm.结果大黄素在9.67～77.36 ng、大黄酚在21.25～172.20 ng范围内呈良好的线性关系,平均回收率为94.60%,RSD=1.8%(n=6).结论所用方法简便、灵敏、准确,可用于回生口服液的质量控制.OBJECTIVE To establish a method for the content determination of emodin and chrysophanol in Huisheng oral solution. METHODS An HPLC method was used, which consisted of Akzonobel Kromasil C18 column（ 150 mm×4.6mm, 5μm）, methanol - 0.07% phosphorie acid（70： 30）as mobile phase, with detection wavelength of 254 nm. RESULTS The linear range of emodin was 9.67 - 77.36 ng （ r=0.9996） and that of chrysophanol was 21.25 - 172.20 ng （ r = 0.9998） .The average recovery was 94.6% ,with RSD of 1.8%. CONCLUSION The method is simple, sensitive and accurate. It can be used to control the quality of Huisheng oral solution.

关 键 词：大黄素 大黄酚 HPLC 回生口服液 

分 类 号：R927.2[医药卫生—药学]