HPLC测定滇黄芩中黄芩苷的含量  

Determination of baicalin content in Scutellaria amoena C.H. Wright by HPLC

作　　者：苏健[1] 向东[1] 张赟华[1] 张雯洁[1] 

机构地区：[1]云南省药品检验所,云南昆明650011

出　　处：《华西药学杂志》2005年第4期356-358,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立高效液相色谱法测定滇黄芩药材中黄芩苷的含量.方法色谱柱为Agilent Zorbax SB-C18柱(4.6 mm×150 mm,5 μm),甲醇-0.2%磷酸(45∶55)为流动相,柱温25℃,流速1 ml·min-1,紫外检测波长280 nm.结果线性范围0.2336～1.1680 μg(r=0.9999),平均回收率为99.13%,RSD=1.13%(n=9).结论所用方法简便、准确、重复性好,可作为滇黄芩药材质量控制的有效方法.OBJECTIVE To determine baicalin content in Scutellaria amoena C. H.Wright. METHODS An HPLC method was adopted, The separation was performed on Zorbax SB- C18column （4.6mm×150mm, 5μm） with a mobile phase of methanol - 0.2% phosphoric acid （45：55）. The flow rate was 1.0 ml·min^-1 and the detective wavelength was 280nm. RESULTS The linearity of the method was well within the range of 0. 2336 to 1.1680μg, and the average recovery of baicalin was 99.13% with RSD ofl.13%（n=9）, CONCLUSION The method is simple, accurate and reliable. It can be used for quality control of Scutellaria amoena C. H. Wright.

关 键 词：高效液相色谱法 滇黄芩 黄芩苷 

分 类 号：R927.2[医药卫生—药学]