棉属（Gossypiumspp．）种子SOD酶的凝胶电泳图谱与种间关系  

Relations Among Gossypiun Species Based on Superoxide DismutaseIsozyme Zymogram

作　　者：周宝良[1] 陈松[1] 沈新莲[1] 黄骏麟[1] 

机构地区：[1]江苏省农业科学院经济作物研究所

出　　处：《江苏农业学报》1995年第1期11-15,共5页Jiangsu Journal of Agricultural Sciences

摘　　要：棉属２９个野生种和４个栽培种４７份材料的种子ＳＯＤ同工酶的凝胶电泳图谱表明：①同一栽培种多数品种的谱带完全一致；②同一染色体组内种间迁移率相同的酶带较多，其中Ｄ染色体组内差异最小，Ｃ染色体组内差异最大。根据研究结果推测：①Ｂ染色体组可能是棉属中最原始的类群，Ａ、Ｅ染色体组由Ｂ染色体组分别进化而来；②雷蒙德氏棉和瑟伯氏棉可能分别为阔叶型和披针叶型异源四倍体棉Ｄ染色体亚组的供体；③Ａ１２０可能属于棉属；④Ａ１１２与奈尔逊氏棉、澳洲棉的亲缘关系较近，与斯托提棉、鲁滨逊氏棉的关系较远。ＳＯＤ酶可以作为研究种间关系和分类的重要方法之一。Polyacrylamide gel electrophoresis of superoxide dismutase(SOD)isozymes was conducted with 47 entries belong to 33 species/races in Gossypium,in order to search for more evidence for affinities between Gossypium species and the origin of the tetraploid species.The SOD zymogram showed that among the varieties of the same cultivated species(G.barbadense and most of G.hirsutum)there were no pronounced differences.The species of the same genome had more bands with similar migration rates,compared with those of different genomes.The most similar bands were found in the D genome and the least in the C genome.In the D genome,the band patterns of G.thurberi and G.raimondii were most similar to those of lanceolate-leaf species(G.darwinii and G.lanceolatum)and latifolious species,respectively.The similarity in migration rates of SOD bands indicates a close affinity among A, B,and E genomes.Based on cytological and biochemical results,we think that the B genome may be the most primitive group in Gossypium.In the C genome,G.australe,G.nelsonii and Alll(undenominated)had similar migration rates,showing their closest relation in the genome. G.bickii(G.genome) had a distinctive pattern,showing its rather distant relation with the C genome.The characteristic band pattern of Gossypium was also found in A120, so it is suggested that A120 possibly belong to Gossypium.

关 键 词：棉属 SOD酶 种间关系 凝胶电泳图谱 棉花 

分 类 号：S562.032[农业科学—作物学]