致病性口腔白色念珠菌耐药株和药物敏感株的ITS基因型比较  

Comparison of ITS genotypes between antifungal drug sensitive and antifungal drug resistant pathogenic oral C.albicans isolates

作　　者：周曾同[1] 赵民[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔内科,上海200011

出　　处：《临床口腔医学杂志》2005年第8期498-502,共5页Journal of Clinical Stomatology

基　　金：上海市卫生局科技发展基金项目(044005)

摘　　要：目的:探讨致病性口腔念珠菌ITS1-ITS2区域进化中变异的序列是否与接受放化疗相关,是否与菌株对抗真菌药物的敏感性相关。方法:收集两组(头颈部肿瘤放化疗患者合并念珠菌感染组和单纯口腔念珠菌感染组)临床菌株,依据NCCLS的M27-A2标准方案测定两组菌株对四种抗真菌药物的最低抑菌浓度;提取菌株的DNA,PCR扩增ITS1-ITS2区域,比较两组ITS1-ITS2序列的差异并比较药物敏感株和耐药株该区域核苷酸碱基序列的差异。结果:全部菌株对5-氟胞嘧啶敏感,92.1%对两性霉素B敏感,放化疗组和单纯白念感染组对氟康唑的耐药比例分别为26.7%和8.7%,对伊曲康唑的耐药比例分别为40%和13%;菌株ITS1-ITS2序列的差异与分组和药物敏感性无明显相关。结论:致病性口腔念珠菌ITS1-ITS2区域进化中变异的序列与是否接受放化疗无明显相关,与菌株对抗真菌药物的敏感性无明显相关。Objective：To compare sequence changes of ITS1-ITS2 regions of pathogenic oral C. albicans isolated from two groups （head and neck cancer patients treated with radiothrapy or chemotherapy and Oral Candidasis patients） ; To compare sequence changes of ITS1-ITS2 regions of antifungal drug sensitive isolates and antifungal drug resistant isolates. Method： The in vitro susceptibilities of 38 oral C. albicans to amphoteritinB（AraB）, 5-flucytosine （5-FC）, itraconazole （ICZ） and fluconazole were determined by NCCLS M27-A2 method. Using PCR amplification of ITS1-ITS2 regions and sequencing analysis to compare sequence changes of ITS1-ITS2 regions of the two groups and explored sequence changes of ITS1-ITS2 regions between antifungal drug sensitive isolates and antifungal drug resistant isolates. Result： All strains were sensitive to 5-flucytosine,92.1% stains were sensitive to arnphoteritin 13, 26.7% and 40% stains isolated from head and neck cancer patients treated with radiothrapy or chemotherapy were resistant to fluconazole and itraconazole respectively, 8.7% and 13% stains isolated from Oral Candidasis patients were resistant to fluconazole and itraconazole respectively ; sequence changes of ITS1-ITS2 regions of stains hadn＇t obvious correlation with antifugal susceptibility and radiothrapy or chemotherapy. Conclusion：Sequence changes of ITS1-ITS2 regions during evolution of pathogenic oral C. albicans hadn＇t obvious correlation with antifugal susceptibility and radiothrapy or chemotherapy.

关 键 词：口腔白色念珠菌 药物敏感性实验 ITS基因型 

分 类 号：R378[医药卫生—病原生物学]