血压平逆转二肾一夹肾性高血压大鼠左室重构的作用及机制的研究  

Reverse Effect of Xueyaping NG on Left Centricle Remodeling Resulting from Renal Hypertension Induced by 2K1C in Rats

作　　者：胡有志[1] 石杰[1] 屈松柏[1] 向楠[1] 柯于鹤[1] 冯德勋[1] 李大锋[1] 

机构地区：[1]湖北中医学院附属医院

出　　处：《中西医结合心脑血管病杂志》2005年第8期703-706,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：湖北省教育厅自然基金项目(No.99A072)

摘　　要：目的研究血压平逆转二肾一夹(2K1C)肾性高血压大鼠左室重构的作用及其机理。方法建立2K1C肾性高血压大鼠(RHR)模型,随机分为血压平大剂量组[73.00g/(kg·d)];血压平中剂量组[36.50g/(kg·d)];血压平小剂量组[18.25g/(kg·d)];依那普利组[10mg/(kg·d)];模型组及假手术对照组。左室及室间隔称重(LVW),计算左室重量指数(LVW/BW)之比,测量心肌细胞形态参数及原癌基因C-myc和C-fos表达阳性细胞的平均光密度值。结果血压平降低血压,LVW、LVW/BW、心肌细胞体积及血清PCⅢ含量,抑制原癌基因C-myc和C-fos的表达,血压平大、中剂量组LVW、LVW/BW及血清PCⅢ含量明显低于模型组(P<0.01),图像分析心肌细胞形态参数(面积、周长、平均直径、长径及短径)及C-myc、C-fos表达阳性的心肌细胞平均光密度值明显低于模型组(P<0.01),与假手术组接近(P>0.05)。结论复方中药血压平具有逆转2K1C肾性高血压大鼠左室重构的作用,降低血压,降低血清PCⅢ含量,抑制Ⅲ型胶原合成,防治心肌纤维化及降低原癌基因C-myc和C-fos的表达,抑制心肌细胞肥大,减轻左心室肥厚,是其逆转二肾一夹肾性高血压大鼠左室重构的部分机制。Objective To investigate the reverse effect of Xueyaping on left ventricle remodeling resulting from renal hypertension induced by 2K1C in rats and to explore the mechanism of Xueyaping. Methods Renal hypertension inrat was induced by 2K1C. Forty rats were divided into 5 groups randomly, group 1 ： large dosage Xueyaping 73 g/（kg·d）; group 2： medium dosage Xueyaping 36.5mg/（kg·d）; group 3. low dosage Xueyaping 18.25 mg/（kg·d）; group 4：Enalapril 10 mg/（kg·d） ;group 5.model group. By the end of eighth week, the body weight was measured and 2 ml blood was taken. The left ventricle, interventricular septal weight （LVW） and body weight（BW） were measured. LVW/BW ratio was calculated. Serum PC Ⅲ was measured. Results The blood pressure was low for rats treated by Xueyaping. The LVW, LVW/BW, heart muscle cell volume and serum PCⅢ were decreased for rats treated by Xueyaping. Xueyaping decrease the expression of C - myc and C - fos. SBP, LVW, LVW/BW and serum PCⅢ in group 1 and 2 were significantly lower than those of group 5 （P〈0.01）. The parameter of heart muscle cells （ Area, circle length,mean diameter, length of long and short axes） and mean light density of positive C- myc and C- fos expression cell in group 1 and 2 were significantly lower than that of group 5 （P 〈 0.01 ）. Conclusion Xueyaping may reverse left ventricle remodeling in hypertensive rats induced by 2K1C. The lowering blood and serum PCⅢ,decreasing the synthesis of type Ⅲ collagen, preventing fibrosis of heart muscle and decreasing the expression of C- myc and C- fos, decreasing left ventricle hypertrophy,might be the partial mechanism of Xueyaping to reverse left ventricle remodeling in hypertensive rats induced by 2K1C.

关 键 词：血压平 2K1C肾性高血压大鼠 左室重构 

分 类 号：R544.1[医药卫生—心血管疾病] R289.5[医药卫生—内科学]