肝移植术后HLA的监测  

Detection of Sensitization to Histocompatibility Antigens after Liver Transplantation

作　　者：李艳[1] 张业伟[2] 

机构地区：[1]河南中医学院第一附属医院检验科,郑州450000 [2]苏州大学第一附属医院普外科

出　　处：《中国中西医结合外科杂志》2005年第4期297-299,共3页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

摘　　要：目的:探讨肝移植受致敏与移植物功能的关系。方法:采用酶联免疫吸附法(ELISA)对25例患者肝移植前后的群体反应性抗体(PRA)进行动态监测。结果:25例患者肝功能的主要指标,包括丙氨酸转氨酶、总胆红素、直接胆红素等大多在术后1周内恢复正常。25例中有1例术前中致敏,术后发生轻度排斥反应1次,经治疗后逆转,其PRA在观察期内一直保持阳性。另外24例受者移植前、后PRA均阴性(PRA<5%),其中1例发生排斥反应1次,但PRA仍保持阴性。结论:肝移植受者的致敏状态与术后的排斥反应密切相关。Objective To investigate the sensitization to HLA antigens and relationship between sensitization and allograft function after liver transplantation Methods The panel reactive antibody（PRA） in serum was determined by using LAT ELISA kit in 25 recipients before and after liver transplantation. Results Moderate sensitization was detected in one of 25 cases before transplantation. The patient experienced one episode of mild rejection which was reversed by treatment with methylprednisolone. RPA in this patient maintained positive for 4 months post transplantation. The remaining 24 patients had negative PRA（ 〈 0.05） before and after surgery, One recipient developed acute rejection, but the PRA was continually negative. Conclusion The sensitization rate in Chinese liver vecipients is low, which might be related to the low incidence of rejection after liver transplantation.

关 键 词：肝移植 群体反应抗体 免疫法 移植物排斥 

分 类 号：R657.3[医药卫生—外科学]