东北鹤虱胶囊制剂的药效学研究  

Pharmaco Dynamic Study of Lappula Echinate Gilib Capsule

作　　者：高卫真[1] 高建华[1] 窦淑兰[2] 孟林[1] 

机构地区：[1]天津医科大学药理学教研室 [2]天津医科大学成教学院

出　　处：《中国中西医结合外科杂志》2005年第4期347-350,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：天津市自然科学基金项目(003608511)

摘　　要：目的:研究东北鹤虱胶囊制剂对兔离体肠平滑肌收缩活动的影响及其对实验性腹泻小鼠的止泻作用。方法:取禁食24h的大耳白兔回肠段,置于37℃台氏液中,通入O2和CO2混合气体,观察东北鹤虱胶囊制剂引起的平滑肌张力变化。小鼠分5组,实验组分别灌胃给予东北鹤虱胶囊制剂及等量蒸馏水,每天给药1次,连续7d。于最后一次给药后1h,除空白对照组外各组小鼠灌胃番泻叶混悬液,观察小鼠的腹泻率、稀便率。结果:东北鹤虱胶囊制剂可剂量依赖性地使回肠平滑肌自律性收缩幅度下降,并可对抗ACh和5-HT所致的回肠收缩,使回肠收缩变规律。使实验性腹泻小鼠稀便率与腹泻指数显著改善。结论:东北鹤虱胶囊制剂可抑制回肠平滑肌的自律性收缩,也可对抗ACh和5-HT引起的回肠平滑肌张力增加,并呈明显的剂量依赖性。对衰竭的回肠平滑肌收缩活动具有双向调节作用。对实验性小鼠腹泻具有较强的抗腹泻作用。Objective To study the effects of Lappula Echinate Gilib（LEG） on smooth muscle contraction of isolated intestine and to observe the antidiarrhea action on mice Methods Rabbits were fasted for 24 h and ileum as obtained and placed in the Tyrode＇s Solution at 37 degrees C with mixed gases of O（2） and CO（2）. The intensity of smooth muscle was observed. 50 mice were randomly divided into 5 groups, mice in experiment groups were administered LEG ig.4,8,16 g/kg and the same volume of distilled water respectively, once a day, for consecutive 7day. One hour after the last dosage, except in control group, all the mice were administered 8% Senna suspension, 0.25 ml/lOg. Diarrhea rate and loose stools rate were observed. Results LEG could decrease regular contraction amplitude of smooth muscle dose-dependently and antagonized contraction of the ileum induced by ACh, 5-HT making it regular; LEG could improve loose stools rate and diarrhea index significantly. Conclusion LEG can inhibit regular contraction of smooth muscle of the ileum; antagonize increment of intensity of the ileum induced by ACh, 5-HT in a dose-dependent way; and can amphiregulate contraction of failure ileum and produce antidiarrhea action.

关 键 词：东北鹤虱胶囊制剂 肠平滑肌 收缩 腹泻 

分 类 号：R285.5[医药卫生—中药学]