吡喹酮对日本血吸虫感染鼠血管活性肠肽和物质P的影响  

Effects of praziquantel on VIP and SP in rats infected with Schistosoma japonicum

作　　者：李多云[1] 周和龙[2] 叶进[2] 蔡淑清[2] 

机构地区：[1]武汉科技大学医学院临床医学院传染科,武汉430062 [2]华中科技大学同济医学院附属协和医院传染科

出　　处：《中国血吸虫病防治杂志》2005年第4期265-267,F0002,共4页Chinese Journal of Schistosomiasis Control

基　　金：湖北省血吸虫病专家咨询委员会2002/2003年度血吸虫病科研项目(A类)~~

摘　　要：目的探讨吡喹酮对日本血吸虫感染小鼠的结肠肌间神经丛中血管活性肠肽(VIP)和物质P(SP)的影响。方法30只昆明小鼠分成2组,每组15只。2组小鼠经皮感染日本血吸虫尾蚴后,治疗组给予吡喹酮500mg/kg,对照组给予生理盐水。应用免疫组织化学技术和计算机彩色病理图文分析系统,观察吡喹酮治疗后日本血吸虫感染小鼠的结肠肌间神经丛内VIP和SP的免疫反应性变化。结果治疗组小鼠结肠肌间神经丛内VIP和SP的免疫反应强度的灰度均值均较对照组显著降低(P<0.05)。结论吡喹酮治疗可以使日本血吸虫感染小鼠的结肠肌间神经丛中VIP和SP含量降低。Objective To study the effects of praziquantel on the expression of vasoactive intestinal peptide （VIP）and substance P （SP）in the myenteric plexus of rats infected with Schistosoma japonicurn. Methods Thirty male rats were divided into 2 groups, every group comprised 15 rats,infected with （30±2） S. japonicum cercaria each. Each rat in the treated group was administered with praziquantel （500 mg/kg）, and in the control group with 0.9% sodium chloride. The immunohistochemical method and colour image analyzer were used to show the immunoreactiveties of VIP and SP in the myenterie plexus of rats infected with Schistosoma japonicum before and after praziquantel treatment. Results After treated with praziquantel, the density and degree of immunostaining of VIP and SP of the treated group significantly reduced compared with the control group （P〈0.05）. Conclusion Praziquantel treatment can significantly reduce the content of VIP and SP in the myenteric plexus of rats infected with Schistosoma japonicum.

关 键 词：吡喹酮 日本血吸虫 肠道神经系统 血管活性肠肽 物质P 

分 类 号：R383.24[医药卫生—医学寄生虫学]