全反式维甲酸对单侧输尿管梗阻大鼠肾脏的作用  

The effect of all-trans-retinoic acid on renal interstitial fibrosis after unilateral ureteral obstruction in rats

作　　者：朱丹[1] 桂保松[1] 卫小红[1] 姚钢炼[1] 高登峰[1] 宁宁[1] 张红莉[1] 

机构地区：[1]西安交通大学医学院第二附属医院肾内科,西安710004

出　　处：《中国血液净化》2005年第7期381-383,共3页Chinese Journal of Blood Purification

基　　金：陕西省西安市科技局社会发展项目基金资助项目(GG0414)

摘　　要：目的研究全反式维甲酸(all-trans-retinoicacid,at-RA)在单侧输尿管梗阻(unilateraluretrealobstructive,UUO)大鼠模型中对肾间质肌成纤维细胞(MyoF)的积聚及胶原Ⅲ(colⅢ)沉积的影响。方法将40只大鼠随机分为假手术组、UUO组、维甲酸大剂量组,维甲酸小剂量组,苯那普利组。后3组分别给予全反式维甲酸20mg、10mg·kg-1·d-1和苯那普利10mg·kg-1·d-1。术后第14天处死各组大鼠,用免疫组化方法测定α平滑肌肌动蛋白(αSMA)及colⅢ的表达。行HE、MASSON染色观察肾脏病理变化。结果全反式维甲酸可显著减少肾间质区MyoF积聚,减轻colⅢ的沉积,并改善肾脏的病理学变化。结论全反式维甲酸可通过减少肾间质区MyoF积聚,减轻colⅢ的沉积而改善UUO所致的肾间质纤维化。Objective To study the effect of all transretinoic acid（at-RA） on the process of renal interstitial fibrosis following unilateral ureteral obstruction （UUO） in rats. Methods Fouty SD rats were randomly assigned into 5 groups ：Two atRA treatment groups （10mg and 20mg·kg^-1·d^-1 respectively）, benazepril treatment group（10mg·kg^-1·d^-1）,UUO control group and normal control group, All of rats were killed 14 days after surgery. Immunohistochemistry was performed on renal tissue to detect smooth muscle actin（α -SMA） which was a cytoskeletal marker of myofibroblast （MyoF） and collagen Ⅲ（col Ⅲ）. Histological studies were also done by he and MASSON stainting. Results Fourteen days after surgery, interstitial myof infiltration and interstitial col Ⅲ deposition were all significantly reduced by at-RA treatment. At-RA treatment also improved the histological changes of UUO rats. Conclusion Reduction of interstitial MyoF infiltration may be an important event by which at RA prevents renal injury caused by UUO.

关 键 词：肾间质纤维化 Α平滑肌肌动蛋白 全反式维甲酸 胶原Ⅲ 肌成纤维细胞 

分 类 号：R692.5[医药卫生—泌尿科学]