芯片显色法检测HBV基因多态性临床应用  

Application of HBV gene polymorphism with DNA chip G

作　　者：郭乃洲 王万相 马达 王惠民[2] 赵建龙[3] 

机构地区：[1]江苏省盐城市第一人民医院检验科,224006 [2]江苏省南通医学院附属医院 [3]中国科学院上海信息与微系统研究所

出　　处：《江西医学检验》2005年第4期313-315,共3页Jiangxi Journal of Medical Laboratory Sciences

基　　金：江苏省"333"工程资助项目(2002年度;项目编号29)

摘　　要：目的用芯片显色法检测HBV DNA在前C/C区、BCP区、P区基因突变来探讨拉米夫丁使用与HBV基因多态性关系。方法用含有HBV DNA前C区1896、1814和BCP区1762、1764以及P区552位点突变信息的探针芯片与HBV DNA杂交,采用显色方法判断结果,检测184例乙型肝炎患者HBV基因多态性,并将拉米夫丁治疗组和非拉米夫丁治疗组HBV基因多态性进行比较。结果184例乙肝患者中,前C区1896、1814和BCP区1762、1764以及P区552位点的突变率分别为28.8%、22.2%、25.5%、29.8%和7.0%;拉米夫丁治疗组和非拉米夫丁治疗组P区552位突变率分别为44.4%和8.7%。结论乙肝患者HBV DNA前C区和BCP区的突变与肝炎慢性化、肝硬化以及HBeAg表达有关;拉米夫丁的使用在HBV DNA P区基因突变中起重要作用。Objective To explore HBV gene polymorphism by detecting HBV gene mutation in pre-C/C,BCP and P region with DNA Chip, acquaint the relation between HBV gene polymorphism and treatment of lamivudine. Methods The probe in chip including mutation information of HBV DNA 1896,1814.1762.1764 ,P552 was hybridized with HBV DNA, estimating results by coloration, 184 cases hepatitis B were detected and the polymorphism of the group treated of lamivudine were compared with another group untreated of lamivudine. Results Among 184 patients of hepatitis B, the mutation frequency of HBV gene on pre-C 1896.1814and BCP 1762.1764 and P 552 was 28.8%.22.2%.25.5%.29.8% and 7.0%, respectively. The mutation frequency of P 552 in the group treated of lamivudine and the group untreated of lamivudine was 44.4% and 8.7%. Conclusions The mutation of HBV DNA pre-C and BCP region is relevant to chronic programming and cirrhosis of hepatitis B, also relevant to the expression of HBeAg ;The treatment of lamivudine plays an important role in mutation of HBV DNA in P region.

关 键 词：芯片 多态性现象 HBV DNA 显色法 拉米夫丁 

分 类 号：R446.62[医药卫生—诊断学] R373.21[医药卫生—临床医学]