吡咯烷二硫代氨基甲酸酯对树突状细胞成熟的影响  

Effect of Nuclear Factor-kappaB Inhibitor Pyrrolidine Dithiocarbamate on Maturation of Dendritic Cells

作　　者：祝恒成[1] 陈江华[2] 何强[2] 刘修恒[1] 程洪涛[1] 

机构地区：[1]武汉大学人民医院泌尿外科,武汉430060 [2]浙江大学医学院附一医院肾病中心

出　　处：《药物流行病学杂志》2005年第1期7-8,58,共3页Chinese Journal of Pharmacoepidemiology

基　　金：武汉大学人才专项基金(502273106)

摘　　要：目的:观察NF-κB抑制剂吡咯烷二硫代氨基甲酸酯(PDTC)对树突状细胞(DC)成熟的影响。方法:利用PDTC作用于体外培养的大鼠不成熟DC,细菌脂多糖(LPS)刺激后,流式细胞仪检测DC共刺激分子CD80、CD86、CD40表达;ELISA分析DC分泌IL-12的变化,NF-κBDNA结合ELISA检测核内NF-κB活性。结果:PDTC抑制DC共刺激分子CD80、CD86、CD40表达;减少DC分泌IL-12;抑制核内NF-κBDNA结合活性。结论:PDTC抑制DC成熟。Objective： To observe the effect of nuclear factor-kappaB （NF-KB） inhibitor pyrrolidine dithiocarbamate（PDTC） on maturation of dendritic cells（DCs）. Method： DCs were prepared from rat bone marrow cells and treated by PDTC, After stimulated by LPS, the cell-surface expression of costimulatory molecules（ CD80, CD86 and CIM0） were analyzed by Flow cytometry, and the production of IL-12 in DCs culture supermatant was determined by ELISA. The activity of NF-κB was investigated by NF-κB binding ELISA, Result： PDTC suppressed cell-surface expression of costimulatory molecules （ CD80,CD86 and CD40） and inhibited the production of IL-12. PDTC could also decrease NF-κB DNA binding activity. Conclusion ： PDTC inhibited maturation of dendritic cells.

关 键 词：吡咯烷二硫代氨基甲酸酯 NF—κB抑制剂 树突状细胞 成熟 

分 类 号：R966[医药卫生—药理学]