红花注射液保存人胎羊膜移植预防肌腱粘连  

THE PREVENTION OF TENDON ADHESION BY TRANSPLANTIANG HUMAN PLACENTAL AMNION PRESERVED IN SAFFLOWER INJECTION: An Experimental Study

作　　者：郝东明[1] 闻辉[1] 于栋[1] 赵文海[1] 

机构地区：[1]长春中医学院,130021

出　　处：《中医正骨》2005年第8期4-5,共2页The Journal of Traditional Chinese Orthopedics and Traumatology

摘　　要：为探讨红花注射液保存的人胎羊膜植入防治肌腱粘连的作用机制。将30只成年鸡随机分成两组,每组15只,各30根屈肌腱予以切断,左足为实验组行断端吻合后植入红花注射液保存的胎羊膜,右足为对照组、空白组分别植入未经药液保存的胎羊膜及单纯吻合。通过肉眼、光镜观察肌腱愈合假鞘形成及粘连情况,并测定生物力学、病理组织学评分及腱组织中羟脯氨酸含量。结果肉眼观察实验组左足爪肌腱完全恢复正常,腱周无粘连,对照组无明显粘连,空白组有粘连,且粘连程度较重。光镜观察实验组腱周有纤维组织形成,腱周裂隙,断端愈合,肌腱外周无明显组织粘连;对照组大致同左足爪,断端基本愈合,腱周无明显粘连;空白组无腱周裂隙形成,有较多成纤维细胞及少量单核细胞浸润。生物力学测定趾关节总屈曲角度、屈趾深肌腱滑移距离比较,实验组明显大于空白组,有极显著性差异(P<0.01);实验组大于对照组,有显著性差异(P<0.05)。病理组织学观察实验组纤维增生量、粘连程度及范围显著小于空白组(P<0.01)。实验组腱内纤维增生量、肌腱粘连程度及范围少于对照组(P<0.05);腱组织中羟脯氨酸含量测定实验组与空白组比较,有显著性差异(P<0.05);实验组与对照组比较,有显著性差异(P<0.05)。表明红花注射液保存的胎羊膜可有效防治肌腱吻合术粘连。In order to study the action mechanism of transplanting human placental amnion preserved in safflower injection (TAPSI) on the prevention of tendon adhesion, 30 fowls were divided into two groups (n=15 each) and 30 flexors tendons of each group were cut off. After the severed-end anastomosis, the left tendons were taken as the experimental group treated by TAPSI, and the right ones were taken as the control group treated by transplanting placental amnion not preserved in the injection and the blank group treated by anastomosis alone; the false sheath formation and tendon adhesion in the tendons were observed by naked eyes and optical microscope; the biomechanical and pathohistological scores and hydroxyproline levels in the tendon tissues were measured. The results included: ① the naked-eyes observations: in the experimental group: completely normal tendons with no adhesion around them; in the control one: no obvious adhesion; but in the blank one: severe adhesion; ② the optical-microscope observations: in the experimental group: fibrous tissue formation around the tendons, peri-tendon cracks and severed-end healing with no significant peri-tendon tissue adhesion; in the control one: roughly the same as the experimental (basic healing of the severed ends with no significant adhesion); in the blank one: no peri-tendon crack formation, many fibroblasts and a few monocytes; ③ the biomechanical measurements: the total flexion angle of the toe joint and slide distance of the profound digital flexor muscle in the experimental group were significantly greater than the ones in the blank (P<0.01) and the ones in the control (P < 0.05); ④ the histopathological observations: the fibrous proliferation, adhesion degree and range in the experimental were obviously less than the ones in the blank (P < 0.01) and the ones in the control (P > 0.05); ⑤ the hydroxyproline measurements: the hydroxyproline level in the tendons of the experimental group had significant differences from the ones of the blank and the control (P

关 键 词：肌腱粘连/治疗 红花注射液/治疗应用 人胎羊膜移植术 实验研究 动物 鸡 

分 类 号：R285[医药卫生—中药学]