HzAm1细胞rpL13基因的序列分析及病毒感染对其转录水平的影响  

Sequence Analysis of RpL13 Gene from HzAm1 Cells and Effect of HaSNPV Infection on the Transcription of RpL13

作　　者：代文涛[1] 邓菲[1] 胡志红[1] 

机构地区：[1]中国科学院武汉病毒研究所病毒学国家重点实验室和无脊椎动物病毒学联合开放实验室

出　　处：《中国病毒学》2005年第4期424-428,共5页Virologica Sinica

基　　金：973项目资助(2003CB114202);中荷战略联盟计划资助(2004CB720404);国家自然科学基金资助(30025003);中国科学院知识创新工程项目资助(Kscx2-1-02;kscx2-SW-301-09);863项目(2003AA214050)

摘　　要：从美洲棉铃虫细胞(HzAM1)中克隆了核糖体大亚基蛋白L13(RibosomalproteinL13,RpL13)的cDNA及其基因组DNA序列,并进行了序列分析。其编码框为666bp,无内含子,预测编码大小约为25kDa的蛋白。通过与其它15种动物的RpL13编码框序列进行进化分析,发现聚类结果与传统物种分类一致。转录研究表明,棉铃虫核型多角体病毒(Helicoverpaarmigerasinglenucleocapsidnucleopolyhedrovirus,HaSNPV)感染HzAm1后使rpL13在细胞内的转录水平降低,在病毒感染后96h,rpL13mRNA的拷贝数降到对照健康细胞的8%。The cDNA and genomic DNA of ribosomal protein L13 （RpL13） gene were cloned from Helicoverpa zea cells （HzAM1）. Sequence analysis indicated that the open reading frame of rpL13 was composed of 666bp without intron,encoding a protein of 25 kDa. The sequence was used to construct phylogentic trees with other known sequences of 15 animal rpL13s. The result suggested it has similar topology with the classical taxonomy. Quantitative RT-PCR assay revealed that the transcription level of rpL13 declined in the HzAml cells infected by Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus （HaSNPV）. At 96 hours post infection, the mRNA copies of rpL13 were about 8% to that of healthy cells.

关 键 词：美洲棉铃虫 核糖体蛋白L13(RpL13) 转录 荧光定量RT—PCR 

分 类 号：Q789[生物学—分子生物学]