猪繁殖与呼吸综合症病毒GP5蛋白原核表达初步研究  

Preliminary Studies of Prokaryotic Expression of GP5 of Porcine Reproductive and Respiratory Syndrome Virus

作　　者：陈勇军[1] 苏鑫铭[1] 高晓飞[1] 郑其升[1] 于春梅[1] 曹瑞兵[1] 周斌[1] 陈溥言[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏南京210095

出　　处：《中国病毒学》2005年第4期441-443,共3页Virologica Sinica

基　　金：国家"863"高技术发展计划资助项目(2001AA249012)

摘　　要：The envelope glycoprotein (GP5) is one of the major structural proteins of Porcine reproductive and respiratory syndrome virus (PRRSV). In this report, the N-terminal hydrophobic sequence of envelope glycoprotein gene (orf5) was deleted by PCR. The gene was subcloned into prokaryotic expressing vector pET-28 a(+) , the recombinant plasmid named pET-GP5 was transformed into E.coli cell BL21. SDS-PAGE and Western-blot indicated that the orf5 gene was expressed successfully and the recombinant fusion protein, which was about 20.8kDa, had immunologically reactive activity. The studies lay foundations for further study on the development of vaccines for PRRSV.The envelope glycoprotein （GP5） is one of the major structural proteins of Porcine reproductive and respiratory syndrome virus （PRRSV）. In this report, the N-terminal hydrophobic sequence of envelope glycoprotein gene （or f5） was deleted by PCR. The gene was subcloned into prokaryotic expressing vector pET-28 a（＋） , the recombinant plasmid named pET-GP5 was transformed into E. coli cell BL21. SDS-PAGE and Western-blot indicated that the or f5 gene was expressed successfully and the recombinant fusion protein, which was about 20.8kDa, had immunologically reactive activity. The studies lay foundations for further study on the development of vaccines for PRRSV.

关 键 词：猪繁殖与呼吸综合症病毒 GP5蛋白 原核表达 

分 类 号：S852.65[农业科学—基础兽医学]