丁型肝炎病毒抗原的表达纯化和抗原性分析  

Expression,Purification and Antigenicity Analysis of the HDV Antigen

作　　者：谢立[1] 黄德庄[1] 贺立香[1] 罗朝霞[1] 周育森[2] 

机构地区：[1]北京市卫生局肝炎研究所 [2]军事医学科学院微生物流行病研究所,北京100071

出　　处：《中国病毒学》2005年第4期444-446,共3页Virologica Sinica

摘　　要：Amplified HDV fragment by RT-PCR was cloned into T vector and PQE_{31} plasmid, which established a prokaryotic expression vector in E coli M_{15} The recombinant protein was purified by Ni-NTA column affinity chromatography, and identified by Western blot and ELISA: HDV antigen was expressed efficiently and had a well antigenicity character. It can be used in HDV clinical diagnosis and epidemiology survey.Amplified HDV fragment by RT-PCR was cloned into T vector and PQE31 plasmid, which established a prokaryotic expression vector in E coli M15 The recombinant protein was purilied by Ni-NTA column affinity chromatography,and identified by Western blot and ELISA：HDV antigen was expressed efficiently and had a well antigenicity character. It can be used in HDV clinical diagnosis and epidemiology survey.

关 键 词：丁型肝炎病毒 基因克隆和表达 丁肝抗原 

分 类 号：R392[医药卫生—免疫学]