枳实及其提取物质量评价法的研究  

Quality evaluation of raw materials and extract of Citrus aurantium

作　　者：袁丹[1] 王青[1] 王启隆[1] 谢媛媛[1] 潘英妮[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学中药分析研究室,辽宁沈阳110016

出　　处：《中国药学杂志》2005年第15期1178-1181,共4页Chinese Pharmaceutical Journal

摘　　要：目的建立枳实及其提取物中辛弗林等生物碱类成分和橙皮苷等4种黄酮类成分的综合质量评价法。方法采用高效液相色谱法,以InertsilODS-3(4.6mm×250mm,5μm)色谱柱分别对枳实及其提取物中辛弗林生物碱类成分和新橙皮苷、橙皮苷、柚皮苷、芸香柚皮苷黄酮类成分进行测定。辛弗林:流动相为甲醇-水(7:4)(水中含0.02%磷酸和0.1%十二烷基硫酸钠),检测波长275nm,流速0.6mL·min-1;橙皮苷等4种黄酮类成分:流动相为甲醇-水-冰醋酸(38:58:4),检测波长284nm,流速1.0mL·min-1。结果辛弗林、橙皮苷、柚皮苷分别在0.04～0.64,0.03～0.48和0.03～0.48g·L-1内,线性关系良好。加样回收率分别为98.3%,98.7%,97.9%。结论本方法准确,重现性好,可作为枳实及其提取物综合质量评价分析法。OBJECTIVE To establish a systematic quality evaluation for the raw materials and the extract of Citrus aurantium. METHODS The contents of synephrine, neohesperidin, hesperidin, naringin and narirutin in the raw materials and the extracts of Citrus aurantium were determined by reversed-phase high performance liquid chromatography with an Inertsil ODS-3（4.6 mm × 250 mm,5 μm）column, respectively. The chromatographic conditions for synephrine included the mobile phase consisting of methanol-water （composed of 0.02% phosphoric acid and 0.1% sodium dodecyl sulfate） （7 ： 4） at the flow rate of 0.6 mL· min^-1, and the detection wavelength at 275 nm. For the determination of 4 flavonone glycosides, a mixture of methanol, water and acetic acid （38：58：4） were used as the mobile phase at the flow rate of 1.0 mL·min^-1, with the detection wavelength at 284 nm. RESULTS The linear ranges were from 0.04 to 0.64 g·L^-1 for synephrine,from 0.03 to 0.48 g·L^-1 for hesperidin, and from 0.03 to 0.48 g·L^-1 for naringin.The recoveries were 98.3%, 98.7% and 97.9%, respectively. CONCLUSION The methods are accurate, reliable, and helpful for the systematic quality evaluation of the raw materials and the extract of Citrus aurantium.

关 键 词：辛弗林 橙皮苷 高效液相色谱法 枳实 枳实黄酮类 质量评价 

分 类 号：R284.1[医药卫生—中药学]