同种异基因大鼠心脏移植急性排斥反应的相关基因分析  

Analysis on acute rejection related genes in rat allogeneic cardiac transplantat ion

作　　者：庄聪文[1] 黄盛东[2] 于伟勇[2] 杨胜生[1] 陈龙[1] 徐志云[2] 张宝仁[2] 

机构地区：[1]南京军区福州总医院心胸外科,福州350025 [2]上海第二军医大学附属长海医院胸心外科研究所

出　　处：《中华器官移植杂志》2005年第8期484-486,共3页Chinese Journal of Organ Transplantation

摘　　要：目的运用基因芯片技术分析同种异基因大鼠心脏移植后急性排斥反应的基因表达谱。方法建立同种异基因大鼠颈部心脏移植模型,并设同种同基因大鼠颈部心脏移植作为对照。术后5d,两组移植心脏中抽提总RNA,纯化后的mRNA进行逆转录制备杂交探针,应用含有4096个靶基因的表达谱芯片对两组移植心脏组织进行差异表达谱分析。结果两组之间杂交信号有明显差异,同种异基因心脏移植术后差异表达基因共有210条(下调96条,上调114条),其中已知基因有33条(下调13条,上调20条),未知基因177条(下调83条,上调94条);已知基因中有15条(上调2条,下调13条)尚未见报道。结论运用基因芯片技术分析同种异基因心脏移植术后急性排斥相关基因,可为进一步研究其发病机理和为治疗提供新思路。Objective To screen acute rejection related genes of allogeneic cardiac transplantation by cDNA microarray technique and study the mechanism of acute rejection of allogeneic cardiac transplantation in rats. Methods Cardiac transplantation model was established in rats neck and rats were derided into syngenic cardiac transplantation group and allogeneic cardiac transplantation control group. RNA was extracted from the transplanted heart and mRNA was purified for reverse transcription probe to analyze difference of gene expression and to screen acute rejection related genes on the 5th day by DNA microarray technique with 4096 target genes. Results 210 acute rejection related genes were different between the groups （96 genes downregulated, 114 genes upregulated）. 33 genes have been known （13 genes downregulated, 21） genes upregulated） and 177 genes have not been known （83 genes downregulated, 94 genes upregulated）, and 15 known genes （2 genes downregulated, 13 genes upregulated） kept unreported. Conclusion Microarray technique for analyzing allogeneic cardiac transplantation rejection genes is a new method for investigating acute rejection mechanism and its therapy.

关 键 词：同种异基因心脏 心脏移植 急性排斥反应 大鼠 基因表达 

分 类 号：R654.2[医药卫生—外科学]