莴苣授粉前后柱头与花柱中钙的分布变化  

THE DYNAMICS OF CALCIUM DISTRIBUTION IN STIGMA AND STYLE OF LETTUCE (LACTUCA SATIVA L.) BEFORE AND AFTER POLLINATION

作　　者：邱义兰[1] 刘如石[1] 谢潮添[2] 杨延红[2] 谷力[2] 田惠桥[2] 

机构地区：[1]湖南师范大学生命科学学院,长沙410081 [2]厦门大学生命科学学院,厦门361005

出　　处：《实验生物学报》2005年第4期277-286,共10页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金(30170060)

摘　　要：莴苣柱头呈两裂片状,有接受花粉的接受面和非接受面之分。授粉前后,柱头接受面乳突细胞的细胞壁中贮存丰富的细小钙颗粒,而非接受面的表皮细胞壁中的钙颗粒很少。在花柱组织中钙的分布具有明显特征,在同一水平上,钙主要分布在引导组织的质外体中,如细胞壁和细胞间隙中,而在引导组织外的薄壁组织中钙主要分布在的细胞内液炮和细胞壁中以及维管束导管内。在花柱不同水平上,花柱中的钙呈现出梯度分布,顶端各组织中的钙颗粒较少,基部各组织中的钙颗粒较多。授粉后1h花柱基部组织的钙颗粒增多,钙梯度分布现象增强。花柱引导组织中的钙梯度分布很可能是吸引花粉管向下生长的原因。讨论了莴苣花柱引导组织中钙梯度分布特征和花粉管在其体内生长的关系。Potassium antimonite was used to deposit calcium in the stigma and style of lettuce（Lactuca sativa L.） before and after pollination. The stigma of lettuce is two splits. Abundant calcium granules are displayed in the wall of papillae on the receptive surface of stigma before and after pollination, which may facilitate pollen germination. However, a few calcium granules in the wall of epidermis cell on no-receptive surface. Calcium distribution in style presents a gradient in transmitting tissue and parenchyma cells from the top to the base of the style before pollination.After pollination, calcium in transmitting tissue distinctly increased and its gradient distribution became more evident. Pollen tubes grow in the intercellular gaps of transmitting tissue. When pollen tubes grew into transmitting tissue, calcium granules in parenchyma around transmitting tissue decreased, suggesting a calcium movement was controlled by pollen tubes. The calcium gradient distribution also appeared in the trachea of vascular bundle of style. In general, calcium in style displays a feature of time-special distribution： transmitting tissue doesn＇t need much more calcium that is only stored in the parenchyma before pollination. However, calcium in parenchyma cells may be transported to transmitting tissue and make the latter contain more calcium to form an evident calcium gradient and meet the requirement of pollen tubes directionally growing after pollination. This is the second sample of calcium gradient existing in style, which was found by using potassium antimonite method.

关 键 词：莴苣 授粉前 授粉后 柱头 花柱 钙 分布 焦锑酸盐沉淀法 花粉管 雌蕊组织 定向生长 

分 类 号：Q944.4[生物学—植物学] S636.2[农业科学—蔬菜学]