利用工程酶改进有机合成的研究进展  

Research Advances in Engineered Enzymes for Improved Organic Synthesis

作　　者：张作良[1] 王凡业[1] 刘均洪[1] 

机构地区：[1]青岛科技大学化工学院,山东青岛266042

出　　处：《上海化工》2005年第8期30-34,共5页Shanghai Chemical Industry

摘　　要：修饰酶用于有机合成已经涉及了许多领域。它包括改变酶反应机制来催化新的反应,改变底物专一性、扩大底物专一性、改善底物专一性,例如动力学拆分中的对映选择性。酶修饰可以通过理性设计或随机突变方法来实现,前者需要知道酶的结构,后者则需要进行筛选。两种策略的酶工程都能成功,并且对于改进在催化中应用的酶非常有意义。最近,有例子表明这两种策略已经应用于许多酶中。Recent developments to modify enzymes for use in organic synthesis have targeted several areas. These include altering the reaction mechanism of the enzyme to catalyse new reactions, switching substrate specificity, expanding substrate specificity, and improving substrate specificity, such as enantioselectivity in kinetic resolutions. Such modifications can be achieved either by rational redesign, which requires knowledge of the enzyme structure, or by random mutagenesis methods followed by screening. Both strategies of enzyme engineering can be successful and are very useful for improving the utility of enzymes for applied catalysis. Several examples illustrating these concepts in a variety of enzyme classes have appeared recently.

关 键 词：工程酶 定向进化 理性设计 

分 类 号：Q814[生物学—生物工程]