Tween-20与牛血清白蛋白的相互作用  

Interaction between Tween-20 and BSA

作　　者：刘静[1] 徐桂英[1] 刘军[1] 于丽[1] 张洪林[2] 于秀芳[2] 

机构地区：[1]山东大学胶体与界面化学教育部重点实验室 [2]曲阜师范大学化学系,山东曲阜273165

出　　处：《物理化学学报》2005年第8期862-866,共5页Acta Physico-Chimica Sinica

基　　金：山东省自然科学基金(Q2003B01);中国博士后科学基金资助项目~~

摘　　要：通过表面张力和等温滴定量热方法,研究了非离子表面活性剂Tween-20与牛血清白蛋白(BSA)的相互作用.结果表明,BSA与Tween-20的相互作用较弱,当Tween-20浓度增大时,能将吸附于表面上的BSA分子取代.BSA/Tween-20混合体系的性质随温度的变化趋势与单一非离子型表面活性剂相似.BSA浓度在3.7×10-5～11.1×10-5kg·L-1范围内,Tween-20的cmc不随BSA浓度的变化而变化.当Tween-20的含量一定时,Tween-20与BSA相互作用过程的热效应也与BSA的浓度无关.BSA/Tween-20混合体系的热力学参数表明,BSA的存在使Tween-20的cmc减小,体系的熵变增大.The interaction between Tween-20 and BSA was studied by surface tension and isothermal titration microcalorimetry. The results show that there is a weak interaction between Tween-20 and BSA and the Tween-20 molecules can replace BSA molecules adsorbed on the surface. A competition adsorption between Tween-20 and BSA occured at the surface of mixed solution. The tendency of change of the properties of the BSA/Tween-20 mixed system with temperature is similar to that of the single non-ionic surfactant. In the range of BSA concentration from 3.7×10^-5kg·L^-1 to 1.1×10^-5 kg·L^-1, the cmc of Tween-20 does not change with BSA concentrations. When the amount of Tween-20 is fixed, the thermal effects of the interaction between BSA and Tween-20 does not depend on the BSA concentration. The thermodynamic parameters of the mixed solutions in the presence or absence of BSA indicate that the cmc of Tween-20 decreases and the value of entropy change of the system increases in the presence of BSA in solution.

关 键 词：Tween一20 BSA 表面张力 等温量热滴定 相互作用 

分 类 号：O629.73[理学—有机化学]