传染性喉气管炎新城疫鸡痘重组病毒免疫效力的研究  

Efficiency of the Recombinant Fowlpox Virus Expressing gB Gene of Larngotracheitis Virus and Fusion Gene of Newcastle Disease Virus

作　　者：智海东[1] 王云峰[1] 张晶[1] 王玫[1] 王凤雪[1] 童光志[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,哈尔滨150001

出　　处：《畜牧兽医学报》2005年第8期807-811,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"863"计划资助(2001AA2130412003AA213020)

摘　　要：在表达鸡传染性喉气管炎病毒(ILTV)糖蛋白gB基因和新城疫病毒(NDV)F基因的重组鸡痘病毒(rF-PV-gB-F)安全性检验合格后,以5.0×101～5.0×104PFU不同含量按0.1 mL/鸡的剂量免疫100只30日龄SPF鸡,30 d后分组分别用ILTVWG株和NDVF48E9株强毒进行攻击.免疫鸡抗鸡痘病毒抗体都转为阳性,痘反应和接种剂量有关,重组疫苗的最小反应剂量为50PFU.重组疫苗可以诱发对新城疫和传染性喉气管炎的保护,0.1mL/鸡的接种量在500～5 000PFU浓度范围内的免疫效果最好,对于ILTV攻击的发病保护率在70%以上,对NDV强毒攻击的抗死亡保护率可以达到80%,这为进一步考察疫苗的免疫效力试验以及进行田间试验奠定了基础.The safety of recombinant fowlpox virus （rFPV-gB-F） expressing glycoprotein B （gB） gene of infectious larngotracheitis virus （ILTV） and F gene of Newcastle disease Virus（NDV） were evaluated. 100 30-day-old SPF chickens were divided into five groups, and inoculated with 50～5×10^4 PFU/0.1 mL rF-PV-gB-F, respectively. 30 days after inoculation, chickens were divided and challenged with ILTV of Wanggang strain and NDV of F48E9 strain, respectively. Antibody titer to FPV was all positive in a dose dependent manner. And in the all range of all the inoculation, it can protect chickens against the challenge of the virulent ILTV and NDV, 7/10 chickens can be protected from clinical symptom in all the inoculation group, 8/10 of chickens inoculated survived from lethal challenge of NDV F48E9 in 500-5000PFU inoculation group, all these results lay the foundation for the further evaluation the efficiency of rFPV-gB-F.

关 键 词：传染性喉气管炎 新城疫 重组鸡痘病毒 最佳免疫剂量 安全性 

分 类 号：S852.65[农业科学—基础兽医学] Q784[农业科学—兽医学]