TNF-α对培养大鼠脂肪细胞凋亡的影响  

Effects of TNF-α on Rat Adipocyte Apoptosis

作　　者：林亚秋[1] 陈国柱[1] 卢建雄[1] 杨公社[1] 

机构地区：[1]西北农林科技大学动物脂肪沉积与肌肉发育实验室,杨凌712100

出　　处：《畜牧兽医学报》2005年第8期823-827,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家教委博士点基金资助项目(1998071232)

摘　　要：以5、101、5ng/mL3种剂量TNF-α作用体外培养的大鼠脂肪细胞0、12、24、36h和48h,采用荧光染色计数、流式细胞仪检测脂肪细胞凋亡率;透射电镜观察脂肪细胞超微结构变化;琼脂糖凝胶电泳检测DNA断裂情况。结果表明,TNF-α对脂肪细胞具有诱导凋亡作用,凋亡率在一定范围内具有剂量和时间依赖性,以10ng/mLTNF-α作用36h为最佳;电镜下观察可见脂肪细胞形态改变,出现染色质凝聚、边集和凋亡小体形成等特征性变化;DNA电泳检测可见处理24h以后出现“梯状”条带,作用0h和12h未见“梯状”条带。The rat adipocytes were treated with 5,10,15 ng/mL tumor necrosis factor α （TNF-α） for 0,12, 24, 36 and 48 hours respectively. The apoptotic rates were determined by means of fluorescent staining and flow cytometry. Ultrastructure of adipocytes was observed by transmission electronic microscopy （TEM） and the DNA fragments were analyzed through agarose gel electrophoresis. The results showed that TNF-α induced the apoptosis of adipocytes and the apoptotic indexes were dependent on the concentration and time. Thus the optimum concentration and time for TNF-α inducing apoptosis was determined at the level of 10 ng/mL and 36 hours. Under the electronic microscope,the characteristics of apoptosis, such as the chromatin condensed and marginated ,and apoptotic bodies were observed in adipocytes. DNA ladders appeared in 24 hours after treatment, whereas,the ladders did not appeared in 0 and 12 hours treatment groups.

关 键 词：TNF-Α 脂肪细胞 凋亡 

分 类 号：S852.2[农业科学—基础兽医学]