HBV感染者血清中HBVcccDNA、HBeAg及HBV DNA的关系  

Relationships of HBVcccDNA with HBV DNA and HBeAg in the subjects'sera with Hepatitis B virus infection

作　　者：吕其军[1] 魏秀桂[1] 聂伟 李毅[1] 田永刚[1] 黄黎[1] 

机构地区：[1]滨州医学院附属胜利油田中心医院,山东东营257034 [2]东营市人民医院,山东东营257000

出　　处：《临床肝胆病杂志》2005年第4期202-203,共2页Journal of Clinical Hepatology

摘　　要：探讨HBV感染者血清HBVcccDNA与血清HBV DNA及HBeAg的关系。分别以PCR分子信标技术和ELISA方法对非HBV相关肝炎、HBV健康携带者、急性乙型肝炎(AHB)、慢性乙型肝炎(CHB)、乙肝肝硬化、乙肝患者血清中HBVcccDNA HBV DNA含量及HBeAg进行了检测。HBVcccDNA仅见于HBVDNA阳性血清中;HBeAg阳性组的HBVcccDNA阳性率显著高于HBeAg阴性组(P<0.05):145例HBVDNA阳性患者中,HBVcccDNA阳性组HBVD- NA水平显著高于HBVcccDNA阴性组(P<0.01)。血清HBVcccDNA可能是乙肝病毒在患者体内大量复制的血清标志。To learn the relationships of covalently closed circular DNA（cccDNA） of hepatitis B virus （HBV） with HBV DNA and HBeAg in patients＇sera with HBV infection. PCR - molecular - beacon technique, which is capable of differentiation of HBV viral genomic DNA and cccDNA, was used to quantify HBV DNA and to detect cccDNA and ELISA method was used to test HBeAg. 23 no - HBV - related hepatitis （ NHB ）, 25 asymptomatic carrier（ ASC ）, 28 acute hepatitis B （AHB）, 94 chronic hepatitis B （CHB）, 32 HBV related liver cirrhosis（ HBV - LC）, 26 HBV related hepatecellular carcinomas（ HBV- HCC） were detected and their cccDNA were 0,0,2,38,10,6 respectively, which was only detected from the patients＇sera with HBV DNA positive cccDNA positive ratio in HBeAg positive group was of much higher than HBeAg negative group （P 〈 0.05）,and of 145 HBV DNA positive subjects,HBV DNA level of cccDNA positive group was higher than that of cccDNA negative group （ P 〈 0.01 ）. The occurrence of cccDNA in the sera may be an important clinical parameter of HBV replication.

关 键 词：肝炎病毒 乙型 共价闭合环状DNA 血清学 

分 类 号：R512.6[医药卫生—内科学]