卡瓦胡椒RAPD反应体系的建立  

Study on the RAPD Amplification System of Kava

作　　者：辛莉[1] 施江[2] 

机构地区：[1]河南科技大学食品工程学院,河南洛阳471003 [2]河南科技大学农学院,河南洛阳471003

出　　处：《生物技术》2005年第4期32-34,共3页Biotechnology

基　　金："十五"国家重点攻关课题资助(编号:2001BA707B02)

摘　　要：卡瓦胡椒RAPD分子标记的研究,目前国内外尚未见有报道。该试验通过CTAB法提取卡瓦胡椒基因组DNA,通过对模板DNA用量、Mg2+浓度、退火温度、电泳上样量等几个单因子试验来建立RAPD稳定扩增体系和反应条件,RAPD扩增结果重复性好,稳定可靠,为卡瓦胡椒RAPD分子标记的研究打下基础。The RAPD molecular markers research of Piper m, methysticum, Piper nigrum and its close relatives have not been reported. Firstly, we extracted Kava＇s genomic DNA from its young leaf; then we did several one- facter tests, such as variant template DNA, variant Mg^2＋ concentration, variant annealing temperature, variant loading, etc., in order to set up the RAPD amplification conditions. The results showed that the reaction was reliable.

关 键 词：卡瓦胡椒 基因组DNA 聚合酶链式反应 随机扩增多态性DNA 

分 类 号：Q789[生物学—分子生物学]