幽门螺杆菌外膜蛋白25基因的克隆及序列分析  

Cloning and sequencing of outer membrane protein 25 of Helicobacter pylori

作　　者：李良仁[1] 王继德[1] 白杨[1] 王群英[1] 

机构地区：[1]南方医科大学南方医院消化病研究所,广东广州510515

出　　处：《中国微生态学杂志》2005年第4期241-243,共3页Chinese Journal of Microecology

基　　金：国家自然科学基金资助(C0302050302)

摘　　要：目的克隆幽门螺杆菌(Helicobacterpylori,Hp)外膜蛋白25(OMP25)基因,并对其进行序列分析。方法利用PCR技术扩增OMP25基因,并将其定向插入pET-22b(+)载体中,以DNA自动序列分析仪进行核苷酸分析。结果DNA序列分析表明,所克隆的OMP25基因序列与GeneBank公布的一致。结论该研究获得了序列正确的幽门螺杆菌OMP25基因,为其重组表达及其相关研究奠定了良好基础。Objective To obtain the outer membrane protein 25（OMP25） gene of Helicobacter pylori （Hp） and clone it into plasmid pET-22b（＋） for nucleotide sequencing. Methods The OMP2S DNA was amplified by PCR. The PCR products were inserted directionally into vector pET-22b （＋） to construct recombinant clones of OMP25 and sequenced. Results The recombinant plasmid was constructed. DNA sequence analysis showed the sequence of OMP25 was the same as that of the published by GenBank, Conclusion A confirmed OMP25 gene had been obstained,thus a good foundation for recombination, expresslon and associated research laid.

关 键 词：螺杆菌 幽门 外膜蛋白25 序列分析 

分 类 号：R378.992[医药卫生—病原生物学]