双歧杆菌DNA对巨噬细胞MAPK的影响  

Influence of Bifidobacterium DNA on MAPK of macrophages

作　　者：王玉林 王立生 朱惠明 朱忠生 马晓冬[2] 

机构地区：[1]暨南大学医学院深圳市人民医院消化科,广东深圳518020 [2]南方医科大学基础部中心实验室,广东广州510515

出　　处：《中国微生态学杂志》2005年第4期256-257,共2页Chinese Journal of Microecology

基　　金：广东省自然科学基金(04006970);深圳市科技局立项课题(200304019)

摘　　要：目的探索青春型双歧杆菌的DNA对巨噬细胞丝裂素活化的蛋白激酶(MAPK)活性的影响。方法以激光共聚焦显微镜定量测定小鼠腹腔巨噬细胞MAPK家系中ERK1/2、JNK和p38的含量。结果双歧杆菌DNA注射组小鼠腹腔巨噬细胞ERK1/2的平均荧光强度明显高于对照组(P<0.01),而JNK和p38的平均荧光强度在2组间则差异无显著性(P>0.05)。结论青春型双歧杆菌的DNA能提高巨噬细胞ERK1/2的活性,这可能是其激活巨噬细胞的途径之一。Objective To explore the influence of DNA of Bifidobacterium adolescence on mitogen-activated protein kinase （MAPK） of macrophages. Method The content of ERK 1/2,JNK and p38 in the family of MAPK of mouse peritoneal macrophages was detected by using laser confocal microscope quantitatively. Result The average fluorescent strength of ERK 1/2 produced by mouse peritoneal macrophages in Bifidobacterium DNA injection group was markedly higher than that in control group（P〈0. 01）. The average fluorescent strength of JNK and p38 did not exit siginificant differences between the two groups（P〉0.05）. Conclusions DNA of Bifi-dobacterium adolescence could promote the activity of ERK 1/2 of macrophages. It was one of the pathways of DNA of Bifidobacterium adolescence activing macrophages possibly.

关 键 词：双歧杆菌DNA 巨噬细胞 丝裂素活化的蛋白激酶 

分 类 号：R378.992[医药卫生—病原生物学]