补中益气汤、党参、白术含药血清对离体脾虚大鼠壁细胞胞内[Ca^(2+)]i的影响  

Effects of the Pi-Invigorating recipe and herbal drugs serum on dynamic changes of the intracellular [Ca^(2+)]i of gastric parietal cells of the Pi-deficiency rats in vitro

作　　者：隋峰[1] 王汝俊[2] 王建华[2] 

机构地区：[1]广州中医药大学 [2]广州中医药大学脾胃研究所,广州510405

出　　处：《中药药理与临床》2005年第3期3-5,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助项目No.30271650

摘　　要：目的:探讨脾虚大鼠的微观病理状态及补脾方药血清对其的调节作用。方法:采用血清药理学方法,以第三代荧光指示剂Fluo-3/AM负载细胞,用共聚焦显微镜观察大鼠单个壁细胞胃泌素刺激后[Ca2+]i的动态变化。结果:睥虚大鼠壁细胞胃泌素刺激后[Ca2+]i升高幅度明显高于正常大鼠,达峰时间也有缩短趋势;补脾方药血清治疗后各组中的各项指标介于两者之间。结论:壁细胞胞内[Ca2+]i的异常变化可能是脾虚证的微观病理机制之一,Ca2+的异常介导可能参与了脾虚证的病理形成;补脾方药血清可不同程度恢复和逆转睥虚状态下的微观病理变化。Objective： To investigate the pathogenic mechanisms of the Pi-deficiency rats in molecular level and effect of the Pi-invigorating recipe and herbal drugs serum on it. Methods： The method of serum phamacology were applied and the dynamic changes of [Ca^2＋ ]i in the gastric parietal cells were measured by confocal microscope with Fluo-3/AM as fluorescence indicator. Results： The percentage of gastrin-induced elevated [Ca^2＋]i magnitude in gastric parietal cells of the Pi-deficiency group was significantly lower than that in gastric parietal cells of the normal group（ P〈 0.05） and the percentage of gastrin-induced elevated [Ca^2＋ ] i magnitude in gastric parietal cells of the Pi-deficiency group were lowered to some extent after addition of the Pi-invigorating recipe and herbal drugs serum. Conclusion： The intracellular calcium of gastric parietal cells of the Pi deficiency rats maybe responsible for the formation of Pi-deficiency in molecular level and the abnormal mediation of the calcium is one of the possible pathogenic mechanisms of Pi-deficiency。

关 键 词：脾虚 壁细胞 [CA^2+]I 补中益气汤 党参 白术 胞内[Ca^2+]i 脾虚大鼠 含药血清 微观病理状态 

分 类 号：R285.5[医药卫生—中药学]