金橙G为电化学探针检测血清白蛋白  

Electrochemical detection of serum albumin with orange G as probe

作　　者：孙伟[1] 韩军英[1] 焦奎[1] 陆路德[2] 

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266042 [2]南京理工大学化工学院,江苏南京210094

出　　处：《化学研究与应用》2005年第4期556-558,共3页Chemical Research and Application

基　　金：国家自然科学基金资助项目(20405008;20375020);青岛市自然科学基金资助项目(04-2-JZ-114);中国博士后科学基金资助项目(2003033492)

摘　　要：An electrochemical quantitative method of serum albumin was developed based on the orange G(OG)as probe.In pH 2.0 Britton-Robinson(B-R)buffer solution,OG has a sensitive reductive peak at -0.27V(vs.SCE)on the hanging mercury drop electrode.After adding human serum albumin(HSA)to the above solution,the reduction peak current of OG decreases apparently without the shift of the peak potential.The decrease of the reduction peak current of OG is in linear with HSA concentration from 4.0 to 28.0mg/L with the regression equation △ip″(nA)=85.02+15.93C(mg/L),γ=0.993.This method was further applied to the determination of the content of HSA in blood samples and the results were in accordance with the traditional Coomassie brilliant blue G-250 spectrophotometric assay.An electrochemical quantitative method of serum albumin was developed based on the orange G（OG） as probe. In pH 2.0 Britton-obinson（B-R）buffer solution, OG has a sensitive reductive peak at -0. 27V（vs.SCE） on the hanging mercury drop electrode. After adding, human serum albumin （HSA） to the above solution, the reduction peak current of OG decreases apparently without the shift of the peak potential. The decrease of the reduction peak current of OG is in linear with HSA concentration from 4.0 to 28.0mg/L with the regression equation Δip＂（nA）=85.02＋15.93C （mg/L）, γ=0.993. This method was further applied to the determination of the content of HSA in blood samples and the results were in accordance with the traditional Coomassic brilliant blue G-250 spectrophotometric assay.

关 键 词：金橙G 人血清白蛋白 伏安法 生物探针 

分 类 号：O657.1[理学—分析化学]