放牧压力下大针茅群体遗传多样性的RAPD分析  

Detecition of Genetic Diversity in Stipa grandis on Grazing Pressure by Using RAPD Markers

作　　者：张红梅[1] 赵萌莉[2] 李青丰[2] 韩冰[3] 杨洁[4] 苏佳楼 张春江 

机构地区：[1]浙江省嘉兴市农业科学院 [2]内蒙古农业大学生态环境学院,呼和浩特010018 [3]内蒙古农业大学生物工程学院,010018 [4]内蒙古大学生命科学院,010021 [5]内蒙古锡林浩特草原站,026000

出　　处：《干旱区资源与环境》2005年第5期185-189,共5页Journal of Arid Land Resources and Environment

基　　金：国家自然基金(30440051);内蒙古科技攻关项目资助

摘　　要：大针茅(Stipa grandis)是我国北方典型草原上最重要的饲用植物之一,分布广,数量大,是优势种之一。本研究应用RAPD技术检测大针茅植物不同放牧压力下群体的遗传多样性,探索放牧强度与遗传分化的相互联系。在放牧和对照群体中,利用18个有效引物共获得了129个位点,其中多态性位点为105个,占81.40%,说明个体发生较高的遗传变异。两个群体都具有特异性位点,说明放牧压力导致某些位点发生了变化,但遗传变异主要存在于群体内(遗传一致度为90.43%)。Stipa grandis is one of the most important forage in typical steppe. It has the broadest distribution in Inner Mongolia. Random Amplified Polymorphic DNA（RAPD） analyses was used to estimate the genetic diversity and explore connection of grazing intensity and genetic variation of S. grandis . A total of 18 primers were used to detected grazing population and CK. The polymorphic products percentage was 81.40% ,testified individual exist very high genetic variation. There were particular loci in two populations. Overgrazing pressure cause some loci change, but genetic variation mostly exist in intra-population（genetic identity is 90.43 % ）.

关 键 词：大针茅 RAPD 遗传多样性 

分 类 号：Q943[生物学—植物学]