水稻白叶枯病菌luxR同源基因的克隆和表达  

Cloning and Expression of a luxR Homologue from Xanthomonas oryzae pv.oryzae

作　　者：梁斌[1] 郭滨[1] 杨晨[1] 张鑫军[1] 戴陆园[2] 余腾琼[2] 沈大棱[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所,上海200433 [2]云南省农科院农作物品种资源站,昆明650205

出　　处：《复旦学报（自然科学版）》2005年第4期540-543,共4页Journal of Fudan University：Natural Science

基　　金：Mcknight基金(14001404);云南省自然科学基金重点资助项目(200C0010Z)

摘　　要：luxR基因是革兰氏阴性菌感应反应(QuorumSensing,QS)LuxR/LuxI环路中起重要作用的基因.从水稻白叶枯病菌中分离到一个luxR同源基因,命名为xooR.xooR全长765bp,编码一个由254个氨基酸残基组成的转录因子,分子质量和等电点分别为28.3ku和8.72.XooR蛋白的N-端15AA-171AA是一个Autoind-bind结构域,C-端191AA-245AA是一个HTH(helix-turn-helix)结合DNA结构域.利用原核表达载体pET-24a(+)和gfp基因构建融合蛋白表达系统,Westernblot分析表明XooR-GFP融合蛋白大小为54ku,xooR基因成功地在E.coliBL21中实现了表达.LuxR homologues are the main component of LuxR/LuxI circus in Quorum Sensing （QS） of Gram-negative bacteria. A luxR homologue was cloned from Xanthomonas. oryzae pv. oryzae, designated xooR. XooR is a transcriptional factor,its full-length spans 765 bp and encoded a putative protein of 254 amino acids, molecular weight and isolectric point are 28.3 ku and 8.72, respectively. The N-terminus froml5 amino acid to 171 amino acid of XooR is autoind-bing domain and the C-terminus 191 amino acid to 245 amino acid is helix-turn-helix DNA-binding domain. The prokaryotic expression vector pET-24a（＋） was used to construct the fusion protein expression system. The vector was subcloned a gfp gene as a marker protein. Western blotting indicated that the XooR-GFP fusion protein is 54 ku. xooR gene was successful expressed as an XooR-GFP fusion protein in E. coli BL21 in vitro.

关 键 词：水稻白叶枯病菌 感应反应 xooR 表达 

分 类 号：Q78[生物学—分子生物学]