丙型肝炎病毒E2基因在转基因番茄植株中的表达  

Expression of Hepatitis C Virus E2 in Transgenic Tomato Plants

作　　者：李凤梅[1] 陈丽珊[2] 崔崇士[1] 杨金水[2] 赵凌侠[3] 刘均洪[4] 任大明[2] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030 [2]复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室,上海200433 [3]上海交通大学农业与生物学院,上海201101 [4]青岛科技大学化工学院,青岛266042

出　　处：《复旦学报（自然科学版）》2005年第4期555-559,共5页Journal of Fudan University：Natural Science

摘　　要：以T-E1E2为模板扩增得到丙型肝炎病毒包膜蛋白基因E2,构建该基因的植物表达载体p35s-E2,通过农杆菌介导的叶盘法转化番茄子叶.转基因番茄植株叶片总DNA的PCR、Southernblot检测结果表明,E2基因已整合进了转基因番茄植株基因组中;RT-PCR,Westernblot分析证实E2基因在转基因番茄植株叶片中表达.Hepatitis C virus envelope protein gene E2 was amplified from the template T-E1 E2 and cloned into a modified plant expression vector to generate a new vector designated p35S-E2. Then Agrobacterium tumefaciens EHA105 containing p35S-E2 were used for leaf disk transformation in tomato. PCR and Southern blot were used to confirm the integration of the E2 gene in the transgenic plant genome. RT-PCR and Western blot analysis showed that the E2 gene was expressed in the transgenic tomato leaf.

关 键 词：丙型肝炎病毒 E2基因 表达 番茄 

分 类 号：Q786[生物学—分子生物学]