肝核转录因子FOXA3相互作用蛋白的初步研究  

Preliminary Identification of the Proteins Interacting with FOXA3 by Yeast Two-hybrid System

作　　者：万兵兵[1] 叶晓霞[1] 石彦[1] 黎莉[1] 施慧莉[1] 霍克克[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室,上海200433

出　　处：《复旦学报（自然科学版）》2005年第4期477-483,共7页Journal of Fudan University：Natural Science

基　　金：国家重点基础研究发展规划资助项目(2001CB510203);十五重大攻关计划资助项目CNHLPP(2004BA711A19)

摘　　要：FOXA3基因是肝核转录因子(HepatocyteNuclearFactor,HNF)基因家族中含有FoxheadBox结构域的3个成员之一,在维持血糖平衡以及调控发育方面发挥着重要的作用.利用FOXA3-AD酵母双杂交载体筛选人肝cDNA-BD酵母双杂交文库,并通过共转化进行验证,获得了两个与FOXA3相互作用的蛋白:C3和TMEM4.这些相互作用蛋白的发现为深入了解FOXA3蛋白参与糖代谢调控的分子机制提供了线索.FOXA3 is one of three member proteins,which belongs to the Hepatocyte Nuclear Factor family and there are Foxbead Box domains in their protein structures. It plays an important role in the maintenance of the blood glucose homoeostasis and regulation of the development of human body. By using FOXA3 as a “bait” to screen a Ga14 BD-fused human liver cDNA library, two “ prey”proteins, TMEM4 and C3, which interacted with FOXA3 were identified. The two protein-protein interactions were further confirmed by co-transforming yeast with FOXA3 and TMEM4,or FOXA3 and C3,respectively. The results provided a clue to explore the mechanism of FOXA3 function in regulation of glucose metabolism.

关 键 词：FOXA3 酵母双杂交 蛋白与蛋白相互作用 人肝cDNA文库 肝核转录因子 

分 类 号：Q71[生物学—分子生物学]