低剂量Con A预刺激抑制足剂量Con A诱导的肝炎发生  

Pretreatment with a low dose of concanavalin A attenuated the development of concanavalin A-induced hepatitis

作　　者：徐旭[1] 魏海明[1] 田志刚[1] 

机构地区：[1]中国科学技术大学生命科学学院免疫学研究所,合肥230027

出　　处：《中国免疫学杂志》2005年第8期563-566,共4页Chinese Journal of Immunology

基　　金："973"项目(2001CB510000);国家自然基金项目(30230340);国家杰出青年科学基金-海外青年学者合作研究基金(30328022)资助

摘　　要：目的:探讨低剂量刀豆蛋白A(ConA)预刺激对后续足剂量ConA诱导的小鼠肝炎的抑制作用。方法:足剂量ConA(15μg/g体重)静脉注射小鼠以诱导ConA肝炎模型。低剂量ConA预刺激组,在诱导ConA肝炎模型前12小时静脉注射低剂量ConA(3μg/g体重);PBS预处理对照组,在诱导ConA肝炎模型前12小时静脉注射PBS。观察两组血清转氨酶(ALT/AST)及肝脏病理变化,同时用流式细胞仪检测肝脏淋巴细胞亚群的比例及绝对数目变化。结果:与PBS预处理相比,低剂量ConA预刺激导致血清转氨酶(ALT/AST)水平显著降低(ALT由3433±788U/L降低到334±68U/L,AST由1358±297U/L降低到167±33U/L),肝脏病理切片显示由ConA引起的肝脏损伤明显减轻,同时伴随着肝脏内CD3+T淋巴细胞比例及绝对数目减少,并且其活化受到明显抑制。结论:低剂量ConA预刺激可以使小鼠抵抗后续的足剂量ConA诱导的肝脏损伤。这种现象与肝脏回流的T淋巴细胞减少及活化程度有关,其细胞与分子机制正在进一步探讨之中。Objective：To investigate the influence of concanavalin A（Con A） pretreagtment on the development of the fulminant murine hepatitis induced by an adequate dose of Con A. Methods： An adequate dose of Con A（ 15μg/g. body. wt） was injected intravenously into C57BL/6 mice to provoke severe liver damage,an extensively-used murine hepatitis medel. A low dose of Con A（3μg/g.bedy.wt） was administrated intravenously 12 h before injection with an adequate dose of Con A. Liver injury was evaluated by serum transaminase assay and H＆E staining. Hepatic lymphoeytes were analyzed by flow cytometry and the absolute amount of lymphocytes per liver was calculated. Results： Pretreatment with a low dose of Con A significantly inhibited Con A-induced liver injury. Both the percentage and the absolute number of liver CD3^＋ T cells in Con A-pretreated mice were lower than those of PBS-pretreated mice. The activation of liver CD3^＋ T cells in Con A-pretreated mice was also prominently inhibited. Concluslon：Con A pretreatrnent exerted the negative effect on the development of Con A-induced hepatitis,which may be result from the decreased recruitment of T ceils into the liver. The underlying mechanisms are under investigation.

关 键 词：刀豆蛋白A(Con A) 血清转氨酶 T淋巴细胞 肝炎 小鼠 

分 类 号：R392.12[医药卫生—免疫学]