体外CIK细胞的细胞因子表达谱研究的初步探讨  

Study on cytokine expression profiles of CIK cells in vitro

作　　者：雷静[1] 钱旻[2] 吴芝莉[1] 张尚权[1] 严缘昌[1] 

机构地区：[1]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031 [2]华东师范大学生命科学院,上海200062

出　　处：《中国免疫学杂志》2005年第8期589-593,共5页Chinese Journal of Immunology

基　　金：上海市科委资助项目(03DZ14104)

摘　　要：目的:探讨CIK细胞的细胞因子表达谱.方法:Ficoll分层液分离人外周血得到非粘附性淋巴细胞,体外经IFN-γ、IL-1β、IL-2及抗CD3单抗诱导分化成CIK细胞.流式细胞术鉴定细胞表型,RT-PCR定性和半定量检测细胞因子的表达.结果:CD3+CD56+细胞百分率均可达50%以上,增殖活性随培养时间的延长而上升,最高集中在14～21天.该诱导条件下获得的CIK细胞不表达IFN-ω、IFN-κ和IFN-λ,其他IFN成员多集中在10～20天高表达,初期和末期都较低;IL家族多数成员在6～10(或20)天时稳定表达,后略有下调;TNF-β和TRAIL各时期组成性表达,表达水平变化很小或几乎不变.TNF-α第20天时高表达.TGF-β1则集中在后期表达,个体差异小.结论:在体外CIK细胞能广泛表达多种类型的细胞因子.Objective：To investigate the cytokine profile expressed by CIK cells. Methods：CIK ceils were induced from peripheral blood mononuclear cells in the presence of IFN-γ, IL-1β, IL-2 and mAb against CD3. The phenotype and characterization of CIK cells were identified by flow eytometric analysis. Cytokine expression profiles were determined at mRNA level by semi-quantitative RT-PCR. Results：The percentage of CD3^＋ CD56^＋ positive ceils reached up to or higher than 50%. CIK ceils couldn＇t express IFN-ω、IFN-κandIFN-λ, under thisk ind of condition. But they could express IFNs principally on day 10 to 20. The level of ILs went down after steadily expressed on day 6 to 10（or 20） .TNF-β and TRAIL constitutively expressed during the culture period in vitro. TNF-α maintained high expression on day 20. TGF-β1mainly expressed on day 30. Conclusion： CIK cells can widely express various kinds of cytokines in vitro.

关 键 词：CIK 细胞因子表达谱 RT-PCR 

分 类 号：R392.11[医药卫生—免疫学]